Results 1 - 6 of 6 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 13349 | 3' | -54.8 | NC_003409.1 | + | 25750 | 0.66 | 0.906218 |
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Target: 5'- uACGCGCUGUCCcccuaagucccgcacGGUCGUucGGGCCCc -3' miRNA: 3'- cUGUGCGACGGGa--------------CUAGUA--UCUGGGu -5' |
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| 13349 | 3' | -54.8 | NC_003409.1 | + | 44620 | 0.67 | 0.877473 |
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Target: 5'- aACAUGCcccucauUGCCCUGGUCAUucaaACCUAc -3' miRNA: 3'- cUGUGCG-------ACGGGACUAGUAuc--UGGGU- -5' |
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| 13349 | 3' | -54.8 | NC_003409.1 | + | 133012 | 0.71 | 0.685339 |
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Target: 5'- gGGCGCGgUGCCUgggGAUCugggcgAGACCCc -3' miRNA: 3'- -CUGUGCgACGGGa--CUAGua----UCUGGGu -5' |
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| 13349 | 3' | -54.8 | NC_003409.1 | + | 97475 | 0.73 | 0.55999 |
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Target: 5'- cAUAUGCUGCCaaGAUCAUuuuGGACCCc -3' miRNA: 3'- cUGUGCGACGGgaCUAGUA---UCUGGGu -5' |
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| 13349 | 3' | -54.8 | NC_003409.1 | + | 21885 | 0.73 | 0.539485 |
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Target: 5'- gGACACGCUgggagcGUCCUGG-CAUAGACCgGa -3' miRNA: 3'- -CUGUGCGA------CGGGACUaGUAUCUGGgU- -5' |
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| 13349 | 3' | -54.8 | NC_003409.1 | + | 130715 | 1.09 | 0.00305 |
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Target: 5'- cGACACGCUGCCCUGAUCAUAGACCCAu -3' miRNA: 3'- -CUGUGCGACGGGACUAGUAUCUGGGU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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