Results 1 - 9 of 9 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 13354 | 5' | -54.8 | NC_003409.1 | + | 54966 | 0.66 | 0.945388 |
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Target: 5'- -gUCUGgccgcaAGGCCGGCCGg---CCACUg -3' miRNA: 3'- agAGACa-----UCUGGUCGGCagcaGGUGG- -5' |
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| 13354 | 5' | -54.8 | NC_003409.1 | + | 25664 | 0.66 | 0.92569 |
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Target: 5'- aUCUCUGUGcACCcaAGUgGUUGUUCaACCg -3' miRNA: 3'- -AGAGACAUcUGG--UCGgCAGCAGG-UGG- -5' |
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| 13354 | 5' | -54.8 | NC_003409.1 | + | 79019 | 0.68 | 0.859043 |
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Target: 5'- cCUCUG-AGAUCAGCCacuccuuGUCGgaacCCACUu -3' miRNA: 3'- aGAGACaUCUGGUCGG-------CAGCa---GGUGG- -5' |
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| 13354 | 5' | -54.8 | NC_003409.1 | + | 98259 | 0.68 | 0.843987 |
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Target: 5'- aCUUgcgAGGucCCAGCCGUCGgcaucaCCGCCa -3' miRNA: 3'- aGAGacaUCU--GGUCGGCAGCa-----GGUGG- -5' |
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| 13354 | 5' | -54.8 | NC_003409.1 | + | 22579 | 0.69 | 0.818802 |
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Target: 5'- gCUaUGUGcGGCCAGCgGUCcugCCACCa -3' miRNA: 3'- aGAgACAU-CUGGUCGgCAGca-GGUGG- -5' |
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| 13354 | 5' | -54.8 | NC_003409.1 | + | 42928 | 0.7 | 0.792074 |
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Target: 5'- gUUUCUGgAGACC-GCCcUCG-CCGCCg -3' miRNA: 3'- -AGAGACaUCUGGuCGGcAGCaGGUGG- -5' |
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| 13354 | 5' | -54.8 | NC_003409.1 | + | 72152 | 0.71 | 0.72503 |
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Target: 5'- aUUUUGUGGGagcguCCGGCCGUC-UCCGCUc -3' miRNA: 3'- aGAGACAUCU-----GGUCGGCAGcAGGUGG- -5' |
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| 13354 | 5' | -54.8 | NC_003409.1 | + | 81331 | 0.72 | 0.685748 |
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Target: 5'- -aUCUGUGGACCAGCUccggaucaagcuuuaUCGUCCugUu -3' miRNA: 3'- agAGACAUCUGGUCGGc--------------AGCAGGugG- -5' |
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| 13354 | 5' | -54.8 | NC_003409.1 | + | 132858 | 1.12 | 0.002508 |
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Target: 5'- uUCUCUGUAGACCAGCCGUCGUCCACCc -3' miRNA: 3'- -AGAGACAUCUGGUCGGCAGCAGGUGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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