Results 1 - 8 of 8 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
13358 | 3' | -49.3 | NC_003409.1 | + | 49224 | 0.66 | 0.995835 |
Target: 5'- cGAAUGCAgCAGUagacgcaggCGCCUAUGaCUAGg-- -3' miRNA: 3'- -CUUGCGU-GUCA---------GUGGAUAC-GAUCaua -5' |
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13358 | 3' | -49.3 | NC_003409.1 | + | 25106 | 0.66 | 0.995103 |
Target: 5'- ---aGCAC-GUCACC-GUGUUAGUGUc -3' miRNA: 3'- cuugCGUGuCAGUGGaUACGAUCAUA- -5' |
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13358 | 3' | -49.3 | NC_003409.1 | + | 109289 | 0.66 | 0.99427 |
Target: 5'- uGACGCACcuuGUCuACCUA-GCUGGg-- -3' miRNA: 3'- cUUGCGUGu--CAG-UGGAUaCGAUCaua -5' |
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13358 | 3' | -49.3 | NC_003409.1 | + | 46788 | 0.67 | 0.988248 |
Target: 5'- cGACGUACuggaGGUCugUaGUGCUGGUGg -3' miRNA: 3'- cUUGCGUG----UCAGugGaUACGAUCAUa -5' |
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13358 | 3' | -49.3 | NC_003409.1 | + | 132966 | 0.67 | 0.986601 |
Target: 5'- cAGCGCGCcGcgCGCCUGUGCgcGUGc -3' miRNA: 3'- cUUGCGUGuCa-GUGGAUACGauCAUa -5' |
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13358 | 3' | -49.3 | NC_003409.1 | + | 23560 | 0.69 | 0.9664 |
Target: 5'- aAugGCGCGGUCGCCUA-GCUcGa-- -3' miRNA: 3'- cUugCGUGUCAGUGGAUaCGAuCaua -5' |
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13358 | 3' | -49.3 | NC_003409.1 | + | 119450 | 0.69 | 0.9664 |
Target: 5'- aAugGCGCGGUCGCCUA-GCUcGa-- -3' miRNA: 3'- cUugCGUGUCAGUGGAUaCGAuCaua -5' |
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13358 | 3' | -49.3 | NC_003409.1 | + | 134857 | 1.06 | 0.013989 |
Target: 5'- cGAACGCACAGUCACCUAUGCUAGUAUc -3' miRNA: 3'- -CUUGCGUGUCAGUGGAUACGAUCAUA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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