Results 1 - 20 of 24 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
1336 | 3' | -53.2 | NC_001335.1 | + | 19846 | 0.65 | 0.819795 |
Target: 5'- uGGCCGGCcCGAagaugauccacaccUUgGGgaacUGCAGCUCGg -3' miRNA: 3'- -CCGGUUGuGCU--------------AAgCC----ACGUCGAGUg -5' |
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1336 | 3' | -53.2 | NC_001335.1 | + | 13016 | 0.66 | 0.792563 |
Target: 5'- aGGCgGauagacaaucgggGCGCGGUgUCGGUcGCucgcGCUCACg -3' miRNA: 3'- -CCGgU-------------UGUGCUA-AGCCA-CGu---CGAGUG- -5' |
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1336 | 3' | -53.2 | NC_001335.1 | + | 50738 | 0.66 | 0.783507 |
Target: 5'- aGGcCCAGCgACGAgucgUCGGccggggGCGGCgCACc -3' miRNA: 3'- -CC-GGUUG-UGCUa---AGCCa-----CGUCGaGUG- -5' |
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1336 | 3' | -53.2 | NC_001335.1 | + | 13226 | 0.67 | 0.762918 |
Target: 5'- cGGCCAucGCACGGUUCcagGUAGC-CAa -3' miRNA: 3'- -CCGGU--UGUGCUAAGccaCGUCGaGUg -5' |
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1336 | 3' | -53.2 | NC_001335.1 | + | 4169 | 0.67 | 0.738551 |
Target: 5'- gGGCCAG-ACGGccUUCGGgccguccccugacgUGUAGCUCAa -3' miRNA: 3'- -CCGGUUgUGCU--AAGCC--------------ACGUCGAGUg -5' |
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1336 | 3' | -53.2 | NC_001335.1 | + | 50600 | 0.67 | 0.73101 |
Target: 5'- cGGCCAcgucggGCACGGUUCGGcacaAGCU-ACg -3' miRNA: 3'- -CCGGU------UGUGCUAAGCCacg-UCGAgUG- -5' |
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1336 | 3' | -53.2 | NC_001335.1 | + | 8913 | 0.68 | 0.698171 |
Target: 5'- gGGUCGAUcucgGCGugcAUUCGGguggGCGGCUCGa -3' miRNA: 3'- -CCGGUUG----UGC---UAAGCCa---CGUCGAGUg -5' |
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1336 | 3' | -53.2 | NC_001335.1 | + | 14297 | 0.68 | 0.687075 |
Target: 5'- cGGUCGGCuACGuguacgUCGGUGCuGCagGCa -3' miRNA: 3'- -CCGGUUG-UGCua----AGCCACGuCGagUG- -5' |
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1336 | 3' | -53.2 | NC_001335.1 | + | 23048 | 0.68 | 0.675923 |
Target: 5'- cGCCGGCACGGccaaCGGUGCugagaAGUUCGa -3' miRNA: 3'- cCGGUUGUGCUaa--GCCACG-----UCGAGUg -5' |
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1336 | 3' | -53.2 | NC_001335.1 | + | 8802 | 0.68 | 0.664728 |
Target: 5'- cGGCCGugAacguaaGcgUCGGUGUAGCcCAg -3' miRNA: 3'- -CCGGUugUg-----CuaAGCCACGUCGaGUg -5' |
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1336 | 3' | -53.2 | NC_001335.1 | + | 47059 | 0.68 | 0.664728 |
Target: 5'- cGCUu--GCGGUUaCGGUGCGGCUgCGCc -3' miRNA: 3'- cCGGuugUGCUAA-GCCACGUCGA-GUG- -5' |
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1336 | 3' | -53.2 | NC_001335.1 | + | 24113 | 0.69 | 0.642254 |
Target: 5'- aGGUCuccGCACaGAgcggCGGUGCuggcGCUCGCg -3' miRNA: 3'- -CCGGu--UGUG-CUaa--GCCACGu---CGAGUG- -5' |
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1336 | 3' | -53.2 | NC_001335.1 | + | 42338 | 0.7 | 0.549478 |
Target: 5'- cGGCCGaccgGCGCGAgcCGcGUGCgucucggcguccaaGGCUCGCu -3' miRNA: 3'- -CCGGU----UGUGCUaaGC-CACG--------------UCGAGUG- -5' |
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1336 | 3' | -53.2 | NC_001335.1 | + | 32084 | 0.7 | 0.541799 |
Target: 5'- aGGCCAGCAgG--UCGGUGCugaccgagcuggAGCgUCACc -3' miRNA: 3'- -CCGGUUGUgCuaAGCCACG------------UCG-AGUG- -5' |
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1336 | 3' | -53.2 | NC_001335.1 | + | 24419 | 0.71 | 0.530894 |
Target: 5'- cGGCCGAC-CGAUcUUGGcGUAGC-CACg -3' miRNA: 3'- -CCGGUUGuGCUA-AGCCaCGUCGaGUG- -5' |
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1336 | 3' | -53.2 | NC_001335.1 | + | 39635 | 0.71 | 0.530894 |
Target: 5'- uGCCGuaGCGuucccaguCGGcUCGGUGCAGCUCGg -3' miRNA: 3'- cCGGU--UGU--------GCUaAGCCACGUCGAGUg -5' |
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1336 | 3' | -53.2 | NC_001335.1 | + | 34377 | 0.71 | 0.520069 |
Target: 5'- uGGCCccuUGCGGc-CGGaUGCGGCUCACg -3' miRNA: 3'- -CCGGuu-GUGCUaaGCC-ACGUCGAGUG- -5' |
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1336 | 3' | -53.2 | NC_001335.1 | + | 33895 | 0.71 | 0.495514 |
Target: 5'- cGCC-GCAUGggUUGGUGCagaccacccgguugGGCUCACg -3' miRNA: 3'- cCGGuUGUGCuaAGCCACG--------------UCGAGUG- -5' |
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1336 | 3' | -53.2 | NC_001335.1 | + | 32684 | 0.71 | 0.477708 |
Target: 5'- cGGCCAACgGCGGcggCGGUggccagcaGCAGuCUCGCg -3' miRNA: 3'- -CCGGUUG-UGCUaa-GCCA--------CGUC-GAGUG- -5' |
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1336 | 3' | -53.2 | NC_001335.1 | + | 17165 | 0.73 | 0.3893 |
Target: 5'- cGGCCAggucgcagGCGCGGUgc--UGCAGCUCGCu -3' miRNA: 3'- -CCGGU--------UGUGCUAagccACGUCGAGUG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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