Results 1 - 7 of 7 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position |
R_P_ratio #
|
P value |
| Predicted miRNA align pattern | |||||||
| 1336 | 5' | -61.8 | NC_001335.1 | + | 24723 | 0.66 | 0.359933 |
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Target: 5'- aGCucCGGGUGCGCCGUggcgCuucccGCGUGGGg -3' miRNA: 3'- -CGc-GCUCGCGCGGCGa---Gu----CGCACCUa -5' |
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| 1336 | 5' | -61.8 | NC_001335.1 | + | 43166 | 0.66 | 0.376764 |
|
Target: 5'- cCGCGAGCGCGUacaCGCcgAGC-UGGAa -3' miRNA: 3'- cGCGCUCGCGCG---GCGagUCGcACCUa -5' |
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| 1336 | 5' | -61.8 | NC_001335.1 | + | 42347 | 0.68 | 0.25082 |
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Target: 5'- gGCGCGAGcCGCGUgCGuCUCGGCGUc--- -3' miRNA: 3'- -CGCGCUC-GCGCG-GC-GAGUCGCAccua -5' |
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| 1336 | 5' | -61.8 | NC_001335.1 | + | 17025 | 0.72 | 0.137737 |
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Target: 5'- cGUGcCGAGCGUGCuCGC-CAGCGUcuGGAa -3' miRNA: 3'- -CGC-GCUCGCGCG-GCGaGUCGCA--CCUa -5' |
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| 1336 | 5' | -61.8 | NC_001335.1 | + | 24201 | 0.73 | 0.110562 |
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Target: 5'- cCGCGAGCGCcagcaccGCCGCUCuGUGcGGAg -3' miRNA: 3'- cGCGCUCGCG-------CGGCGAGuCGCaCCUa -5' |
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| 1336 | 5' | -61.8 | NC_001335.1 | + | 34273 | 0.74 | 0.102115 |
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Target: 5'- -gGCGGuGUGCGCCGgUCGGCGcGGAUg -3' miRNA: 3'- cgCGCU-CGCGCGGCgAGUCGCaCCUA- -5' |
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| 1336 | 5' | -61.8 | NC_001335.1 | + | 5344 | 1.06 | 0.000326 |
|
Target: 5'- uGCGCGAGCGCGCCGCUCAGCGUGGAUc -3' miRNA: 3'- -CGCGCUCGCGCGGCGAGUCGCACCUA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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