Results 21 - 29 of 29 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 13360 | 3' | -52.6 | NC_003409.1 | + | 4589 | 0.69 | 0.907739 |
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Target: 5'- gAGAAAGAgGACGcGUaGGCCAgGUGCUGg -3' miRNA: 3'- -UCUUUCU-CUGC-CAgUCGGU-CAUGGCg -5' |
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| 13360 | 3' | -52.6 | NC_003409.1 | + | 80087 | 0.7 | 0.865621 |
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Target: 5'- cAGucuGGAGACGGa-GGCCAucgACCGCa -3' miRNA: 3'- -UCuu-UCUCUGCCagUCGGUca-UGGCG- -5' |
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| 13360 | 3' | -52.6 | NC_003409.1 | + | 3866 | 0.7 | 0.857791 |
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Target: 5'- uGAGAGGGcACGGgCA-CCAGUuuGCCGCc -3' miRNA: 3'- uCUUUCUC-UGCCaGUcGGUCA--UGGCG- -5' |
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| 13360 | 3' | -52.6 | NC_003409.1 | + | 27248 | 0.7 | 0.857791 |
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Target: 5'- uAGAAuuuGGACGGgCGGUgGGUGCUGCu -3' miRNA: 3'- -UCUUuc-UCUGCCaGUCGgUCAUGGCG- -5' |
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| 13360 | 3' | -52.6 | NC_003409.1 | + | 20182 | 0.71 | 0.824385 |
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Target: 5'- cGGAAAGGGACGuggagugcgcGUCAGCUGcaGCCGUa -3' miRNA: 3'- -UCUUUCUCUGC----------CAGUCGGUcaUGGCG- -5' |
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| 13360 | 3' | -52.6 | NC_003409.1 | + | 30966 | 0.71 | 0.81555 |
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Target: 5'- uGGAGgcgGGGGugGGuUCAGCgGGUggugcgccaacuACCGCg -3' miRNA: 3'- -UCUU---UCUCugCC-AGUCGgUCA------------UGGCG- -5' |
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| 13360 | 3' | -52.6 | NC_003409.1 | + | 116774 | 0.71 | 0.806539 |
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Target: 5'- gAGAGGGAGACGcagCGGCCGGgugcgACgGUa -3' miRNA: 3'- -UCUUUCUCUGCca-GUCGGUCa----UGgCG- -5' |
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| 13360 | 3' | -52.6 | NC_003409.1 | + | 134270 | 0.72 | 0.776627 |
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Target: 5'- cGAGAGAGACGGgauguggaaauaCAGaCGGUGCuCGCu -3' miRNA: 3'- uCUUUCUCUGCCa-----------GUCgGUCAUG-GCG- -5' |
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| 13360 | 3' | -52.6 | NC_003409.1 | + | 9996 | 1.12 | 0.003485 |
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Target: 5'- cAGAAAGAGACGGUCAGCCAGUACCGCu -3' miRNA: 3'- -UCUUUCUCUGCCAGUCGGUCAUGGCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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