Results 1 - 9 of 9 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 13364 | 5' | -60.7 | NC_003409.1 | + | 38627 | 0.66 | 0.633272 |
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Target: 5'- aGGCgccgcAGUCGUCCGCUcugacGCGGACUGc -3' miRNA: 3'- cUCGau---UCGGCGGGCGAc----CGCCUGAU- -5' |
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| 13364 | 5' | -60.7 | NC_003409.1 | + | 115651 | 0.66 | 0.612915 |
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Target: 5'- cGGGCUAGuuccgguauGCCGCUCGCgggugcggUGGUGGugUc -3' miRNA: 3'- -CUCGAUU---------CGGCGGGCG--------ACCGCCugAu -5' |
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| 13364 | 5' | -60.7 | NC_003409.1 | + | 109170 | 0.67 | 0.562396 |
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Target: 5'- gGGGUUcguGGCCGCCgGCUGGgacgcaggcCGGGCg- -3' miRNA: 3'- -CUCGAu--UCGGCGGgCGACC---------GCCUGau -5' |
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| 13364 | 5' | -60.7 | NC_003409.1 | + | 51931 | 0.67 | 0.552411 |
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Target: 5'- uGGGCUAcGgCGCCCuCUGGCGG-Cg- -3' miRNA: 3'- -CUCGAUuCgGCGGGcGACCGCCuGau -5' |
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| 13364 | 5' | -60.7 | NC_003409.1 | + | 32300 | 0.68 | 0.513066 |
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Target: 5'- aGAGCU-GGCauCCagGCUGGCGGACa- -3' miRNA: 3'- -CUCGAuUCGgcGGg-CGACCGCCUGau -5' |
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| 13364 | 5' | -60.7 | NC_003409.1 | + | 127639 | 0.68 | 0.484327 |
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Target: 5'- aGGGUgccuuacGGGCCGCCCGaaacaagugGGCGGGCg- -3' miRNA: 3'- -CUCGa------UUCGGCGGGCga-------CCGCCUGau -5' |
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| 13364 | 5' | -60.7 | NC_003409.1 | + | 6085 | 0.71 | 0.370064 |
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Target: 5'- aGGCcgGAGUCGUCCGUUGGUGGAa-- -3' miRNA: 3'- cUCGa-UUCGGCGGGCGACCGCCUgau -5' |
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| 13364 | 5' | -60.7 | NC_003409.1 | + | 28355 | 0.74 | 0.216908 |
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Target: 5'- uAGCUGcGCUGCaaauCGCUGGCGGACg- -3' miRNA: 3'- cUCGAUuCGGCGg---GCGACCGCCUGau -5' |
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| 13364 | 5' | -60.7 | NC_003409.1 | + | 14051 | 1.06 | 0.001202 |
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Target: 5'- cGAGCUAAGCCGCCCGCUGGCGGACUAc -3' miRNA: 3'- -CUCGAUUCGGCGGGCGACCGCCUGAU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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