Results 1 - 9 of 9 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
13367 | 5' | -54.1 | NC_003409.1 | + | 7738 | 0.67 | 0.901272 |
Target: 5'- -gCCG-CGUUCGCCCaagcauccGGCCAgGUCGa -3' miRNA: 3'- aaGGCaGUAAGUGGGc-------UCGGUgUAGC- -5' |
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13367 | 5' | -54.1 | NC_003409.1 | + | 119664 | 0.7 | 0.797811 |
Target: 5'- -cCCGUCAaUCAaaguuuUCCGAGCCGcCAUUGg -3' miRNA: 3'- aaGGCAGUaAGU------GGGCUCGGU-GUAGC- -5' |
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13367 | 5' | -54.1 | NC_003409.1 | + | 44432 | 0.7 | 0.788523 |
Target: 5'- cUCCGUgGUUacgagCGCUuuuUGGGCCACAUCGg -3' miRNA: 3'- aAGGCAgUAA-----GUGG---GCUCGGUGUAGC- -5' |
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13367 | 5' | -54.1 | NC_003409.1 | + | 15860 | 1.06 | 0.00549 |
Target: 5'- aUUCCGUCAUUCACCCGAGCCACAUCGu -3' miRNA: 3'- -AAGGCAGUAAGUGGGCUCGGUGUAGC- -5' |
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13367 | 5' | -54.1 | NC_003409.1 | + | 23345 | 0.7 | 0.797811 |
Target: 5'- -cCCGUCAaUCAaaguuuUCCGAGCCGcCAUUGg -3' miRNA: 3'- aaGGCAGUaAGU------GGGCUCGGU-GUAGC- -5' |
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13367 | 5' | -54.1 | NC_003409.1 | + | 81389 | 0.69 | 0.824696 |
Target: 5'- cUCCaUCAcUC-CCCGGGCCACAgCGc -3' miRNA: 3'- aAGGcAGUaAGuGGGCUCGGUGUaGC- -5' |
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13367 | 5' | -54.1 | NC_003409.1 | + | 115501 | 0.68 | 0.865729 |
Target: 5'- -aCCGgaacuaGCCCGGGCCACAUUc -3' miRNA: 3'- aaGGCaguaagUGGGCUCGGUGUAGc -5' |
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13367 | 5' | -54.1 | NC_003409.1 | + | 116607 | 0.68 | 0.865729 |
Target: 5'- -aCCGUCg--CACCCGGccGCUGCGUCu -3' miRNA: 3'- aaGGCAGuaaGUGGGCU--CGGUGUAGc -5' |
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13367 | 5' | -54.1 | NC_003409.1 | + | 100018 | 0.66 | 0.93589 |
Target: 5'- -cCCG-CAgaCACCUGAGacCCGCGUCGc -3' miRNA: 3'- aaGGCaGUaaGUGGGCUC--GGUGUAGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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