Results 1 - 8 of 8 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 13368 | 3' | -54.5 | NC_003409.1 | + | 133118 | 0.67 | 0.857346 |
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Target: 5'- -aCCUGCC-UGCGGAuccCGCGGCCGg -3' miRNA: 3'- agGGACGGuACGUUUca-GUGUCGGUa -5' |
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| 13368 | 3' | -54.5 | NC_003409.1 | + | 102851 | 0.67 | 0.849181 |
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Target: 5'- aUCCCUGCCAgGCGcc--CGCuAGCCAg -3' miRNA: 3'- -AGGGACGGUaCGUuucaGUG-UCGGUa -5' |
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| 13368 | 3' | -54.5 | NC_003409.1 | + | 49272 | 0.67 | 0.840802 |
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Target: 5'- gCCCUGCCuaGUggGGUgGCGcGCCAc -3' miRNA: 3'- aGGGACGGuaCGuuUCAgUGU-CGGUa -5' |
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| 13368 | 3' | -54.5 | NC_003409.1 | + | 36460 | 0.68 | 0.786502 |
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Target: 5'- uUCCCUcUCAUGCAccugAAG-CACGGCCGc -3' miRNA: 3'- -AGGGAcGGUACGU----UUCaGUGUCGGUa -5' |
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| 13368 | 3' | -54.5 | NC_003409.1 | + | 46224 | 0.7 | 0.726792 |
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Target: 5'- -aCCUGCCcgGUuuGAGUCAUGGUCAg -3' miRNA: 3'- agGGACGGuaCGu-UUCAGUGUCGGUa -5' |
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| 13368 | 3' | -54.5 | NC_003409.1 | + | 98401 | 0.7 | 0.726792 |
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Target: 5'- -aCCUGCCcuugGCGAAGUUACugaccucccAGCCAUg -3' miRNA: 3'- agGGACGGua--CGUUUCAGUG---------UCGGUA- -5' |
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| 13368 | 3' | -54.5 | NC_003409.1 | + | 83240 | 0.74 | 0.466563 |
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Target: 5'- aUCCCaGgCAUGCAGAGUCGCAGaaGUa -3' miRNA: 3'- -AGGGaCgGUACGUUUCAGUGUCggUA- -5' |
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| 13368 | 3' | -54.5 | NC_003409.1 | + | 16685 | 1.06 | 0.004401 |
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Target: 5'- cUCCCUGCCAUGCAAAGUCACAGCCAUc -3' miRNA: 3'- -AGGGACGGUACGUUUCAGUGUCGGUA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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