Results 1 - 4 of 4 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
13371 | 3' | -48.7 | NC_003409.1 | + | 4314 | 0.66 | 0.997867 |
Target: 5'- aCCUaCAGCaugGCCUGGGAUACcucucaGAGGc- -3' miRNA: 3'- -GGA-GUUGa--CGGACUCUAUGa-----CUUCaa -5' |
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13371 | 3' | -48.7 | NC_003409.1 | + | 99852 | 0.66 | 0.997747 |
Target: 5'- gCCUCGaaaaaucccaagauACUGCUUGAGAgucGCUGGGc-- -3' miRNA: 3'- -GGAGU--------------UGACGGACUCUa--UGACUUcaa -5' |
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13371 | 3' | -48.7 | NC_003409.1 | + | 62975 | 0.66 | 0.997444 |
Target: 5'- gCUCuGCUGCCUGuuaucGAUGCUcGAGa- -3' miRNA: 3'- gGAGuUGACGGACu----CUAUGAcUUCaa -5' |
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13371 | 3' | -48.7 | NC_003409.1 | + | 20898 | 1.08 | 0.013892 |
Target: 5'- cCCUCAACUGCCUGAGAUACUGAAGUUc -3' miRNA: 3'- -GGAGUUGACGGACUCUAUGACUUCAA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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