Results 1 - 9 of 9 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 13371 | 5' | -52.7 | NC_003409.1 | + | 100260 | 0.66 | 0.959187 |
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Target: 5'- aCAGCUCg---UGCCUCA-GCUGAAUa -3' miRNA: 3'- aGUCGGGgucaAUGGAGUaCGACUUA- -5' |
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| 13371 | 5' | -52.7 | NC_003409.1 | + | 35267 | 0.66 | 0.955244 |
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Target: 5'- -uGGgCCCGGUUACCUgcucuuuugCGUGCUGc-- -3' miRNA: 3'- agUCgGGGUCAAUGGA---------GUACGACuua -5' |
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| 13371 | 5' | -52.7 | NC_003409.1 | + | 60950 | 0.66 | 0.95105 |
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Target: 5'- cUUGGCCUCAGggGCCUCGUucGaCUGGc- -3' miRNA: 3'- -AGUCGGGGUCaaUGGAGUA--C-GACUua -5' |
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| 13371 | 5' | -52.7 | NC_003409.1 | + | 133209 | 0.66 | 0.941893 |
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Target: 5'- cCGGCCCCGGUggugaggcACCUgCAggcGCUGGc- -3' miRNA: 3'- aGUCGGGGUCAa-------UGGA-GUa--CGACUua -5' |
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| 13371 | 5' | -52.7 | NC_003409.1 | + | 107910 | 0.66 | 0.941893 |
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Target: 5'- -aAGCCUCAG-UGCCUgCAcGCUGAu- -3' miRNA: 3'- agUCGGGGUCaAUGGA-GUaCGACUua -5' |
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| 13371 | 5' | -52.7 | NC_003409.1 | + | 31851 | 0.69 | 0.872856 |
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Target: 5'- cCAGCCUgCAGcUACUUaCGUGCUGGAg -3' miRNA: 3'- aGUCGGG-GUCaAUGGA-GUACGACUUa -5' |
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| 13371 | 5' | -52.7 | NC_003409.1 | + | 80150 | 0.69 | 0.865066 |
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Target: 5'- gCuGCCCCAGcu-CCUCGUGgCUGGu- -3' miRNA: 3'- aGuCGGGGUCaauGGAGUAC-GACUua -5' |
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| 13371 | 5' | -52.7 | NC_003409.1 | + | 32054 | 0.73 | 0.661595 |
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Target: 5'- aCAGCCCCGGgaGCCaCGUGUgGAGUa -3' miRNA: 3'- aGUCGGGGUCaaUGGaGUACGaCUUA- -5' |
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| 13371 | 5' | -52.7 | NC_003409.1 | + | 20938 | 0.92 | 0.051825 |
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Target: 5'- cUCAGCCCCAGUUACCUCAUG-UGAAUc -3' miRNA: 3'- -AGUCGGGGUCAAUGGAGUACgACUUA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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