Results 21 - 27 of 27 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 13373 | 5' | -55 | NC_003409.1 | + | 39570 | 0.71 | 0.623882 |
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Target: 5'- uGUAGCCCCAAAgaccGUcaaaGCGCcGCAugGCu -3' miRNA: 3'- uCGUCGGGGUUUa---CA----CGCG-CGUugUG- -5' |
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| 13373 | 5' | -55 | NC_003409.1 | + | 107862 | 0.71 | 0.613314 |
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Target: 5'- cGGCAGCCCU--AUG-GUGCGCAAgAa -3' miRNA: 3'- -UCGUCGGGGuuUACaCGCGCGUUgUg -5' |
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| 13373 | 5' | -55 | NC_003409.1 | + | 96857 | 0.72 | 0.581732 |
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Target: 5'- aGGCAGUCuaucgucuCCAgaucacccAGUGUGCGCGCAGCu- -3' miRNA: 3'- -UCGUCGG--------GGU--------UUACACGCGCGUUGug -5' |
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| 13373 | 5' | -55 | NC_003409.1 | + | 115950 | 0.72 | 0.560858 |
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Target: 5'- uGGguGCCuCUggGUGUGC-CGCAAgGCa -3' miRNA: 3'- -UCguCGG-GGuuUACACGcGCGUUgUG- -5' |
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| 13373 | 5' | -55 | NC_003409.1 | + | 24191 | 0.74 | 0.476064 |
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Target: 5'- cAGCAGCCCCcccucgcgcacUGcGCGCGCGGCGg -3' miRNA: 3'- -UCGUCGGGGuuu--------ACaCGCGCGUUGUg -5' |
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| 13373 | 5' | -55 | NC_003409.1 | + | 39661 | 0.77 | 0.330413 |
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Target: 5'- -cCAGCCCCA--UGcUGCGCaGCAACACg -3' miRNA: 3'- ucGUCGGGGUuuAC-ACGCG-CGUUGUG- -5' |
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| 13373 | 5' | -55 | NC_003409.1 | + | 22463 | 1.09 | 0.002543 |
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Target: 5'- gAGCAGCCCCAAAUGUGCGCGCAACACc -3' miRNA: 3'- -UCGUCGGGGUUUACACGCGCGUUGUG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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