Results 1 - 10 of 10 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 13375 | 3' | -64.1 | NC_003409.1 | + | 113763 | 0.66 | 0.532623 |
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Target: 5'- cCGCGCGUcgGGCcGUugGUGGGAaaacCGGc- -3' miRNA: 3'- -GCGCGCG--CCGcCGugCACCCU----GCCua -5' |
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| 13375 | 3' | -64.1 | NC_003409.1 | + | 119964 | 0.66 | 0.504622 |
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Target: 5'- aCGCGCGCGGCgcccacgccGGCGCcUGGcacGCGGc- -3' miRNA: 3'- -GCGCGCGCCG---------CCGUGcACCc--UGCCua -5' |
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| 13375 | 3' | -64.1 | NC_003409.1 | + | 109137 | 0.66 | 0.495429 |
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Target: 5'- gGCGCGUGG-GGUcgaAUGUGGGAagugucaGGAg -3' miRNA: 3'- gCGCGCGCCgCCG---UGCACCCUg------CCUa -5' |
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| 13375 | 3' | -64.1 | NC_003409.1 | + | 19315 | 0.67 | 0.474581 |
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Target: 5'- uGgGUGCGuuuuuccacaugauGCGcCACGUGGGGCGGGc -3' miRNA: 3'- gCgCGCGC--------------CGCcGUGCACCCUGCCUa -5' |
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| 13375 | 3' | -64.1 | NC_003409.1 | + | 20793 | 0.67 | 0.459458 |
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Target: 5'- cCGgGCGCGGCcuGGCGCacagaaGGGAgGGGg -3' miRNA: 3'- -GCgCGCGCCG--CCGUGca----CCCUgCCUa -5' |
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| 13375 | 3' | -64.1 | NC_003409.1 | + | 53224 | 0.69 | 0.338493 |
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Target: 5'- uCGCGCGaCGGCGGCggagcaAUGUaGGGAaaucucagucCGGAUg -3' miRNA: 3'- -GCGCGC-GCCGCCG------UGCA-CCCU----------GCCUA- -5' |
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| 13375 | 3' | -64.1 | NC_003409.1 | + | 52584 | 0.7 | 0.303651 |
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Target: 5'- aGCagGCGUGGCGGC-CaaGGGGCGGGg -3' miRNA: 3'- gCG--CGCGCCGCCGuGcaCCCUGCCUa -5' |
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| 13375 | 3' | -64.1 | NC_003409.1 | + | 87389 | 0.7 | 0.297023 |
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Target: 5'- cCGgGCGCGGgGGCGC-UGGG-CGGu- -3' miRNA: 3'- -GCgCGCGCCgCCGUGcACCCuGCCua -5' |
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| 13375 | 3' | -64.1 | NC_003409.1 | + | 118773 | 0.92 | 0.009511 |
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Target: 5'- aCGCGCGCGGCGGCACG-GaGGACGGAUc -3' miRNA: 3'- -GCGCGCGCCGCCGUGCaC-CCUGCCUA- -5' |
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| 13375 | 3' | -64.1 | NC_003409.1 | + | 24213 | 1.04 | 0.001056 |
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Target: 5'- gCGCGCGCGGCGGCACGUGGGACGGAUc -3' miRNA: 3'- -GCGCGCGCCGCCGUGCACCCUGCCUA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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