Results 1 - 9 of 9 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 13376 | 3' | -46.1 | NC_003409.1 | + | 119178 | 0.65 | 0.999977 |
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Target: 5'- gCCGGGUuuacgcaGCUGCGUAUAc-- -3' miRNA: 3'- -GGCCCAcaauaaaCGGCGCAUAUuac -5' |
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| 13376 | 3' | -46.1 | NC_003409.1 | + | 12486 | 0.66 | 0.999949 |
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Target: 5'- gCGGGUGUacgguuuaUcgUUGCCgacuGUGUGUAAa- -3' miRNA: 3'- gGCCCACA--------AuaAACGG----CGCAUAUUac -5' |
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| 13376 | 3' | -46.1 | NC_003409.1 | + | 72317 | 0.66 | 0.999929 |
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Target: 5'- -gGGGUGUUAUUaacggcaUGCUGCcacUAGUGg -3' miRNA: 3'- ggCCCACAAUAA-------ACGGCGcauAUUAC- -5' |
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| 13376 | 3' | -46.1 | NC_003409.1 | + | 53262 | 0.67 | 0.999909 |
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Target: 5'- uCCGGGUGUcgacggaaUGCCGuCGUuggcaagAGUGg -3' miRNA: 3'- -GGCCCACAauaa----ACGGC-GCAua-----UUAC- -5' |
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| 13376 | 3' | -46.1 | NC_003409.1 | + | 105461 | 0.67 | 0.999844 |
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Target: 5'- uCgGGGUGggugUUGUCGgGUGUGAc- -3' miRNA: 3'- -GgCCCACaauaAACGGCgCAUAUUac -5' |
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| 13376 | 3' | -46.1 | NC_003409.1 | + | 74163 | 0.68 | 0.999581 |
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Target: 5'- uUGGGUGUUGUcgccucUGCCGcCGUAg---- -3' miRNA: 3'- gGCCCACAAUAa-----ACGGC-GCAUauuac -5' |
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| 13376 | 3' | -46.1 | NC_003409.1 | + | 25785 | 0.68 | 0.999342 |
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Target: 5'- cCUGGGUGUUAUgcaccgUGCagguuuucgcaGCGUAUAc-- -3' miRNA: 3'- -GGCCCACAAUAa-----ACGg----------CGCAUAUuac -5' |
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| 13376 | 3' | -46.1 | NC_003409.1 | + | 40268 | 0.77 | 0.891316 |
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Target: 5'- uCCGGGUGccua--GCCGCGUAgAGUGg -3' miRNA: 3'- -GGCCCACaauaaaCGGCGCAUaUUAC- -5' |
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| 13376 | 3' | -46.1 | NC_003409.1 | + | 25971 | 1.11 | 0.019074 |
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Target: 5'- cCCGGGUGUUAUUUGCCGCGUAUAAUGc -3' miRNA: 3'- -GGCCCACAAUAAACGGCGCAUAUUAC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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