Results 1 - 6 of 6 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
13377 | 5' | -59.7 | NC_003409.1 | + | 54824 | 0.66 | 0.746504 |
Target: 5'- -aCGCCCUUgacguaCCUacuggUCaCCUCCGGGUCa- -3' miRNA: 3'- ggGCGGGAA------GGA-----AG-GGAGGCCUAGgc -5' |
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13377 | 5' | -59.7 | NC_003409.1 | + | 68430 | 0.67 | 0.737098 |
Target: 5'- gCCGCCag-CaacCCCUCUGGAUCUa -3' miRNA: 3'- gGGCGGgaaGgaaGGGAGGCCUAGGc -5' |
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13377 | 5' | -59.7 | NC_003409.1 | + | 84965 | 0.68 | 0.679158 |
Target: 5'- cCCCGguCCCggagggUCCUUCUC-CCGGugacUCCGa -3' miRNA: 3'- -GGGC--GGGa-----AGGAAGGGaGGCCu---AGGC- -5' |
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13377 | 5' | -59.7 | NC_003409.1 | + | 123564 | 0.69 | 0.610016 |
Target: 5'- aCCCGCCCUcggga-CUUCUGGAUCCc -3' miRNA: 3'- -GGGCGGGAaggaagGGAGGCCUAGGc -5' |
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13377 | 5' | -59.7 | NC_003409.1 | + | 25873 | 0.69 | 0.580473 |
Target: 5'- cCUCGUCCgUCCcgCCCg-CGGGUCCGa -3' miRNA: 3'- -GGGCGGGaAGGaaGGGagGCCUAGGC- -5' |
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13377 | 5' | -59.7 | NC_003409.1 | + | 26795 | 1.09 | 0.001399 |
Target: 5'- cCCCGCCCUUCCUUCCCUCCGGAUCCGc -3' miRNA: 3'- -GGGCGGGAAGGAAGGGAGGCCUAGGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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