Results 1 - 9 of 9 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 13379 | 5' | -54.2 | NC_003409.1 | + | 117208 | 0.66 | 0.94826 |
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Target: 5'- uGCAGCAGUCCgCCGauauCUUGUaguuggggAGGUuaUCCAu -3' miRNA: 3'- -CGUUGUUAGG-GGC----GAGCG--------UCCA--AGGU- -5' |
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| 13379 | 5' | -54.2 | NC_003409.1 | + | 61387 | 0.66 | 0.939063 |
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Target: 5'- cGCAcCGA-CUCCGUauuaUCGCGGGUcucgUCCAu -3' miRNA: 3'- -CGUuGUUaGGGGCG----AGCGUCCA----AGGU- -5' |
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| 13379 | 5' | -54.2 | NC_003409.1 | + | 8720 | 0.66 | 0.923409 |
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Target: 5'- aGguACAG-CCCCGUUgugaguccUGCGGGUUUCGg -3' miRNA: 3'- -CguUGUUaGGGGCGA--------GCGUCCAAGGU- -5' |
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| 13379 | 5' | -54.2 | NC_003409.1 | + | 19665 | 0.67 | 0.911728 |
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Target: 5'- gGCAACcggGGUCCCC-CUCGUuGGUcuauacaauUCCAu -3' miRNA: 3'- -CGUUG---UUAGGGGcGAGCGuCCA---------AGGU- -5' |
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| 13379 | 5' | -54.2 | NC_003409.1 | + | 97923 | 0.67 | 0.905516 |
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Target: 5'- aGCAGCAgGUCCCUaagauaGCUCGCA--UUCCu -3' miRNA: 3'- -CGUUGU-UAGGGG------CGAGCGUccAAGGu -5' |
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| 13379 | 5' | -54.2 | NC_003409.1 | + | 125645 | 0.67 | 0.892362 |
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Target: 5'- aGCAGCGGgagCCCCaGCa-GCGGGagCCAc -3' miRNA: 3'- -CGUUGUUa--GGGG-CGagCGUCCaaGGU- -5' |
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| 13379 | 5' | -54.2 | NC_003409.1 | + | 73426 | 0.7 | 0.79504 |
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Target: 5'- gGCAACAGUCCUgGCUC-CAGGa---- -3' miRNA: 3'- -CGUUGUUAGGGgCGAGcGUCCaaggu -5' |
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| 13379 | 5' | -54.2 | NC_003409.1 | + | 7656 | 0.71 | 0.747165 |
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Target: 5'- -gAGCAGUCUCUGUcuccgUCGCAGGUggcaUCCGa -3' miRNA: 3'- cgUUGUUAGGGGCG-----AGCGUCCA----AGGU- -5' |
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| 13379 | 5' | -54.2 | NC_003409.1 | + | 33725 | 1.11 | 0.002801 |
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Target: 5'- cGCAACAAUCCCCGCUCGCAGGUUCCAc -3' miRNA: 3'- -CGUUGUUAGGGGCGAGCGUCCAAGGU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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