Results 21 - 28 of 28 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
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R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 13388 | 5' | -58.6 | NC_003409.1 | + | 114427 | 0.75 | 0.246488 |
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Target: 5'- gGC-CCCAGUUuaugcuaagGGCGGUGCUACCgCCAu -3' miRNA: 3'- -UGuGGGUCGA---------UCGUCACGAUGGgGGU- -5' |
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| 13388 | 5' | -58.6 | NC_003409.1 | + | 119226 | 0.67 | 0.672641 |
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Target: 5'- gGCaACCuCAGCUgggaauaccAGCGGa-CUACCCCCAa -3' miRNA: 3'- -UG-UGG-GUCGA---------UCGUCacGAUGGGGGU- -5' |
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| 13388 | 5' | -58.6 | NC_003409.1 | + | 119394 | 0.66 | 0.712235 |
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Target: 5'- uAUACCCAGCUGGgucuaccCAGcUGCguaaACCCCg- -3' miRNA: 3'- -UGUGGGUCGAUC-------GUC-ACGa---UGGGGgu -5' |
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| 13388 | 5' | -58.6 | NC_003409.1 | + | 122186 | 0.66 | 0.714241 |
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Target: 5'- uACACCCAGUguaagaaugucuguGguGUGCUGCgagaCCCUAu -3' miRNA: 3'- -UGUGGGUCGau------------CguCACGAUG----GGGGU- -5' |
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| 13388 | 5' | -58.6 | NC_003409.1 | + | 128072 | 0.68 | 0.621199 |
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Target: 5'- -gGCCCAuGCggGGCucUGCUGCCCUCAc -3' miRNA: 3'- ugUGGGU-CGa-UCGucACGAUGGGGGU- -5' |
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| 13388 | 5' | -58.6 | NC_003409.1 | + | 130759 | 0.67 | 0.662384 |
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Target: 5'- -gGCCCAGCUgGGCAGcaccguCCCCCGc -3' miRNA: 3'- ugUGGGUCGA-UCGUCacgau-GGGGGU- -5' |
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| 13388 | 5' | -58.6 | NC_003409.1 | + | 132656 | 0.68 | 0.600625 |
|
Target: 5'- uCGCCCaacaGGCUGGC-GUGggACCCCUg -3' miRNA: 3'- uGUGGG----UCGAUCGuCACgaUGGGGGu -5' |
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| 13388 | 5' | -58.6 | NC_003409.1 | + | 133698 | 0.75 | 0.271354 |
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Target: 5'- uGCACcgCCAGCggUAGCAG-GuCUGCCCCCAg -3' miRNA: 3'- -UGUG--GGUCG--AUCGUCaC-GAUGGGGGU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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