Results 21 - 28 of 28 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 13388 | 5' | -58.6 | NC_003409.1 | + | 132656 | 0.68 | 0.600625 |
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Target: 5'- uCGCCCaacaGGCUGGC-GUGggACCCCUg -3' miRNA: 3'- uGUGGG----UCGAUCGuCACgaUGGGGGu -5' |
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| 13388 | 5' | -58.6 | NC_003409.1 | + | 14194 | 0.69 | 0.549722 |
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Target: 5'- uGCGCuCCgAGCUGGCAGaGC-ACCCCgAg -3' miRNA: 3'- -UGUG-GG-UCGAUCGUCaCGaUGGGGgU- -5' |
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| 13388 | 5' | -58.6 | NC_003409.1 | + | 24212 | 0.69 | 0.527729 |
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Target: 5'- gGCGCgCCAGCggugGGCGGgGCcccauccucccgGCCCCCAu -3' miRNA: 3'- -UGUG-GGUCGa---UCGUCaCGa-----------UGGGGGU- -5' |
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| 13388 | 5' | -58.6 | NC_003409.1 | + | 87793 | 0.73 | 0.350022 |
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Target: 5'- aGCAgCCAGCUGaauccUGCUGCCCCCAg -3' miRNA: 3'- -UGUgGGUCGAUcguc-ACGAUGGGGGU- -5' |
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| 13388 | 5' | -58.6 | NC_003409.1 | + | 56828 | 0.73 | 0.34224 |
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Target: 5'- gGCACCCugaAGUcgUAGCAGUGUUGCuuaaaCCCCAg -3' miRNA: 3'- -UGUGGG---UCG--AUCGUCACGAUG-----GGGGU- -5' |
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| 13388 | 5' | -58.6 | NC_003409.1 | + | 133698 | 0.75 | 0.271354 |
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Target: 5'- uGCACcgCCAGCggUAGCAG-GuCUGCCCCCAg -3' miRNA: 3'- -UGUG--GGUCG--AUCGUCaC-GAUGGGGGU- -5' |
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| 13388 | 5' | -58.6 | NC_003409.1 | + | 114427 | 0.75 | 0.246488 |
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Target: 5'- gGC-CCCAGUUuaugcuaagGGCGGUGCUACCgCCAu -3' miRNA: 3'- -UGuGGGUCGA---------UCGUCACGAUGGgGGU- -5' |
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| 13388 | 5' | -58.6 | NC_003409.1 | + | 47207 | 1.08 | 0.00146 |
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Target: 5'- aACACCCAGCUAGCAGUGCUACCCCCAu -3' miRNA: 3'- -UGUGGGUCGAUCGUCACGAUGGGGGU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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