Results 1 - 9 of 9 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 13389 | 3' | -56.4 | NC_003409.1 | + | 27289 | 0.66 | 0.83036 |
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Target: 5'- cUGGguuaGGCA-GCUGa--UGCAGCGGCUGc -3' miRNA: 3'- -ACC----CCGUgCGAUaagACGUCGUCGAC- -5' |
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| 13389 | 3' | -56.4 | NC_003409.1 | + | 66547 | 0.66 | 0.812814 |
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Target: 5'- cGGGGCcaaggaaCUAUUCUGCAacaGGCUGg -3' miRNA: 3'- aCCCCGugc----GAUAAGACGUcg-UCGAC- -5' |
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| 13389 | 3' | -56.4 | NC_003409.1 | + | 125493 | 0.67 | 0.794578 |
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Target: 5'- cUGGGGCuccCGCUG--CUGUGGCucccGCUGc -3' miRNA: 3'- -ACCCCGu--GCGAUaaGACGUCGu---CGAC- -5' |
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| 13389 | 3' | -56.4 | NC_003409.1 | + | 31371 | 0.67 | 0.794577 |
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Target: 5'- -aGGGCcacCGCcGUccuccUCUGCAGCAGCa- -3' miRNA: 3'- acCCCGu--GCGaUA-----AGACGUCGUCGac -5' |
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| 13389 | 3' | -56.4 | NC_003409.1 | + | 131270 | 0.7 | 0.586952 |
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Target: 5'- gUGGGGCACGaaaacagGC-GCAGCUGa -3' miRNA: 3'- -ACCCCGUGCgauaagaCGuCGUCGAC- -5' |
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| 13389 | 3' | -56.4 | NC_003409.1 | + | 73032 | 0.71 | 0.570324 |
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Target: 5'- cUGGaGGCAgGCUcaccccgCUGCAuGCGGCUGu -3' miRNA: 3'- -ACC-CCGUgCGAuaa----GACGU-CGUCGAC- -5' |
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| 13389 | 3' | -56.4 | NC_003409.1 | + | 96859 | 0.71 | 0.539485 |
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Target: 5'- gGGGGCACGCU--UCUGgCGGaGGCg- -3' miRNA: 3'- aCCCCGUGCGAuaAGAC-GUCgUCGac -5' |
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| 13389 | 3' | -56.4 | NC_003409.1 | + | 101587 | 0.76 | 0.301501 |
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Target: 5'- gGGuGGCGCGCcggugcggUGUUCUGCGGUGGUUGu -3' miRNA: 3'- aCC-CCGUGCG--------AUAAGACGUCGUCGAC- -5' |
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| 13389 | 3' | -56.4 | NC_003409.1 | + | 47315 | 1.08 | 0.002167 |
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Target: 5'- cUGGGGCACGCUAUUCUGCAGCAGCUGu -3' miRNA: 3'- -ACCCCGUGCGAUAAGACGUCGUCGAC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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