Results 1 - 6 of 6 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 1339 | 3' | -56.6 | NC_001335.1 | + | 21292 | 0.66 | 0.628435 |
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Target: 5'- uGGCUCcggGAGCUGGcUGAUGA-GGAc -3' miRNA: 3'- -UCGAGcuaCUCGACCcGCUACUgCCU- -5' |
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| 1339 | 3' | -56.6 | NC_001335.1 | + | 5853 | 0.66 | 0.595508 |
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Target: 5'- uGGCcagCGGgccGAGCUGGGgGAgagaaccgcUGACGGc -3' miRNA: 3'- -UCGa--GCUa--CUCGACCCgCU---------ACUGCCu -5' |
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| 1339 | 3' | -56.6 | NC_001335.1 | + | 16072 | 0.66 | 0.584583 |
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Target: 5'- gGGCUCGGaGAuCUaGGCGAUGACGc- -3' miRNA: 3'- -UCGAGCUaCUcGAcCCGCUACUGCcu -5' |
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| 1339 | 3' | -56.6 | NC_001335.1 | + | 23843 | 0.68 | 0.509647 |
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Target: 5'- gGGCUCGucgGAGCUGgaGGCGca-GCGGGa -3' miRNA: 3'- -UCGAGCua-CUCGAC--CCGCuacUGCCU- -5' |
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| 1339 | 3' | -56.6 | NC_001335.1 | + | 32902 | 0.74 | 0.221473 |
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Target: 5'- cGGCUCGGgcUGGGCagGGGCGGUGcuccACGGu -3' miRNA: 3'- -UCGAGCU--ACUCGa-CCCGCUAC----UGCCu -5' |
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| 1339 | 3' | -56.6 | NC_001335.1 | + | 6513 | 1.09 | 0.000673 |
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Target: 5'- aAGCUCGAUGAGCUGGGCGAUGACGGAg -3' miRNA: 3'- -UCGAGCUACUCGACCCGCUACUGCCU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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