Results 1 - 9 of 9 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 13391 | 3' | -58.1 | NC_003409.1 | + | 87031 | 0.66 | 0.817161 |
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Target: 5'- aGCUGGaGCGGcgGGagugUGUgUGCCCGGg-- -3' miRNA: 3'- -CGACC-CGCCa-CC----ACAgAUGGGCUgug -5' |
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| 13391 | 3' | -58.1 | NC_003409.1 | + | 28366 | 0.66 | 0.781675 |
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Target: 5'- aCUGGGCGGggGGUG---ACCCaACAUa -3' miRNA: 3'- cGACCCGCCa-CCACagaUGGGcUGUG- -5' |
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| 13391 | 3' | -58.1 | NC_003409.1 | + | 93666 | 0.67 | 0.772453 |
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Target: 5'- aCUGGGCGGUGGguUGUgUGggCGGCGg -3' miRNA: 3'- cGACCCGCCACC--ACAgAUggGCUGUg -5' |
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| 13391 | 3' | -58.1 | NC_003409.1 | + | 18705 | 0.67 | 0.763108 |
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Target: 5'- -gUGGuuGGUGGUGUUUACCuauacccucgCGGCAUg -3' miRNA: 3'- cgACCcgCCACCACAGAUGG----------GCUGUG- -5' |
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| 13391 | 3' | -58.1 | NC_003409.1 | + | 38317 | 0.68 | 0.71485 |
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Target: 5'- --aGGGUGGU-GUGguuaUACCCGACAg -3' miRNA: 3'- cgaCCCGCCAcCACag--AUGGGCUGUg -5' |
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| 13391 | 3' | -58.1 | NC_003409.1 | + | 109846 | 0.69 | 0.654748 |
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Target: 5'- cGCcGcGGCGGgaggcGGUGgc-GCCCGGCGCa -3' miRNA: 3'- -CGaC-CCGCCa----CCACagaUGGGCUGUG- -5' |
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| 13391 | 3' | -58.1 | NC_003409.1 | + | 103635 | 0.69 | 0.634483 |
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Target: 5'- gGCaggGGGCGGcUGGcggGUCggguguCCUGACGCa -3' miRNA: 3'- -CGa--CCCGCC-ACCa--CAGau----GGGCUGUG- -5' |
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| 13391 | 3' | -58.1 | NC_003409.1 | + | 115625 | 0.72 | 0.476661 |
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Target: 5'- gGgUGcGGUGGUGGUGUCUguaAUCUGGCAg -3' miRNA: 3'- -CgAC-CCGCCACCACAGA---UGGGCUGUg -5' |
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| 13391 | 3' | -58.1 | NC_003409.1 | + | 49991 | 1.11 | 0.001142 |
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Target: 5'- uGCUGGGCGGUGGUGUCUACCCGACACa -3' miRNA: 3'- -CGACCCGCCACCACAGAUGGGCUGUG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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