Results 1 - 6 of 6 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
13391 | 5' | -57.6 | NC_003409.1 | + | 82516 | 0.66 | 0.824509 |
Target: 5'- -aGUGUC-GCGCUGCaccgaagcgagCgACCCGCCGu -3' miRNA: 3'- gaCACAGuCGCGACGca---------G-UGGGUGGU- -5' |
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13391 | 5' | -57.6 | NC_003409.1 | + | 70697 | 0.66 | 0.817639 |
Target: 5'- uUGaGUCAGCuGCcGCG-C-CCCACCAg -3' miRNA: 3'- gACaCAGUCG-CGaCGCaGuGGGUGGU- -5' |
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13391 | 5' | -57.6 | NC_003409.1 | + | 74453 | 0.66 | 0.817639 |
Target: 5'- aCUGcaaccgGUCAGgGCgaccucgGCGaCGCCCGCUAa -3' miRNA: 3'- -GACa-----CAGUCgCGa------CGCaGUGGGUGGU- -5' |
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13391 | 5' | -57.6 | NC_003409.1 | + | 88039 | 0.69 | 0.663482 |
Target: 5'- aCUGUGUCAGgGgucuccgGCGUCGCacagaCGCCGg -3' miRNA: 3'- -GACACAGUCgCga-----CGCAGUGg----GUGGU- -5' |
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13391 | 5' | -57.6 | NC_003409.1 | + | 13650 | 0.69 | 0.608124 |
Target: 5'- -cGUGUCAGacuucgcggaggaGCUaGCGUCAaucaCCACCAa -3' miRNA: 3'- gaCACAGUCg------------CGA-CGCAGUg---GGUGGU- -5' |
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13391 | 5' | -57.6 | NC_003409.1 | + | 50025 | 1.08 | 0.00183 |
Target: 5'- aCUGUGUCAGCGCUGCGUCACCCACCAc -3' miRNA: 3'- -GACACAGUCGCGACGCAGUGGGUGGU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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