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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 13392 | 3' | -55.7 | NC_003409.1 | + | 104932 | 0.66 | 0.904221 |
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Target: 5'- aUCCCGCUGCCugcucuggcGGGCggucUUGGUGaggcggUCGUGg -3' miRNA: 3'- -GGGGCGGUGG---------CCCG----AAUCACa-----AGUAC- -5' |
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| 13392 | 3' | -55.7 | NC_003409.1 | + | 18035 | 0.66 | 0.904221 |
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Target: 5'- cUCuuGCuCGCCaGGCUUGGgGUUCAc- -3' miRNA: 3'- -GGggCG-GUGGcCCGAAUCaCAAGUac -5' |
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| 13392 | 3' | -55.7 | NC_003409.1 | + | 36729 | 0.67 | 0.853897 |
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Target: 5'- aCCCGCCGCaacgacuCGGGCUggagagGGUGagaAUGg -3' miRNA: 3'- gGGGCGGUG-------GCCCGAa-----UCACaagUAC- -5' |
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| 13392 | 3' | -55.7 | NC_003409.1 | + | 63661 | 0.71 | 0.657809 |
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Target: 5'- aCCCuuugCGUCACCGGGCUguUGGUGUg---- -3' miRNA: 3'- -GGG----GCGGUGGCCCGA--AUCACAaguac -5' |
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| 13392 | 3' | -55.7 | NC_003409.1 | + | 52180 | 1.11 | 0.002102 |
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Target: 5'- cCCCCGCCACCGGGCUUAGUGUUCAUGg -3' miRNA: 3'- -GGGGCGGUGGCCCGAAUCACAAGUAC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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