Results 1 - 8 of 8 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 13393 | 5' | -56.7 | NC_003409.1 | + | 14341 | 0.66 | 0.84892 |
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Target: 5'- -cUCGGAGcgcAGGCuaCCUgGGGCGUCgACGa -3' miRNA: 3'- aaAGUCUC---UCUG--GGA-CCCGCAGgUGU- -5' |
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| 13393 | 5' | -56.7 | NC_003409.1 | + | 115861 | 0.66 | 0.840747 |
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Target: 5'- -aUCAGAcauGGACCCUGugaGGUGUCUGCc -3' miRNA: 3'- aaAGUCUc--UCUGGGAC---CCGCAGGUGu -5' |
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| 13393 | 5' | -56.7 | NC_003409.1 | + | 37489 | 0.67 | 0.768042 |
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Target: 5'- uUUUCAGAGuuACCCUGagguccucGGCGacuucggcggaggUCCACAa -3' miRNA: 3'- -AAAGUCUCucUGGGAC--------CCGC-------------AGGUGU- -5' |
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| 13393 | 5' | -56.7 | NC_003409.1 | + | 71148 | 0.67 | 0.759368 |
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Target: 5'- --cCAGGGAcgcuaaGaACCCcGGGUGUCCGCGc -3' miRNA: 3'- aaaGUCUCU------C-UGGGaCCCGCAGGUGU- -5' |
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| 13393 | 5' | -56.7 | NC_003409.1 | + | 107499 | 0.7 | 0.615626 |
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Target: 5'- -aUCGGcguuGGAGACgCUuggacggGGGCGUCCGCGg -3' miRNA: 3'- aaAGUC----UCUCUGgGA-------CCCGCAGGUGU- -5' |
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| 13393 | 5' | -56.7 | NC_003409.1 | + | 130896 | 0.71 | 0.534547 |
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Target: 5'- --cCAGGGAGAguaCCUGGGgGUCCGg- -3' miRNA: 3'- aaaGUCUCUCUg--GGACCCgCAGGUgu -5' |
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| 13393 | 5' | -56.7 | NC_003409.1 | + | 78302 | 0.75 | 0.360393 |
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Target: 5'- gUUCAGGGGGGCCUgGGGUcUCCACu -3' miRNA: 3'- aAAGUCUCUCUGGGaCCCGcAGGUGu -5' |
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| 13393 | 5' | -56.7 | NC_003409.1 | + | 56046 | 1.06 | 0.00291 |
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Target: 5'- aUUUCAGAGAGACCCUGGGCGUCCACAu -3' miRNA: 3'- -AAAGUCUCUCUGGGACCCGCAGGUGU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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