Results 1 - 6 of 6 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 13395 | 5' | -55.1 | NC_003409.1 | + | 73025 | 0.66 | 0.894681 |
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Target: 5'- cAGGCUCaCCCC-GCUgcaUGCGGCUg- -3' miRNA: 3'- cUUUGAG-GGGGuUGGag-ACGCCGAaa -5' |
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| 13395 | 5' | -55.1 | NC_003409.1 | + | 80152 | 0.66 | 0.880568 |
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Target: 5'- -uAGCUgCCCCAGCUcCUcGUGGCUg- -3' miRNA: 3'- cuUUGAgGGGGUUGGaGA-CGCCGAaa -5' |
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| 13395 | 5' | -55.1 | NC_003409.1 | + | 20356 | 0.66 | 0.865512 |
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Target: 5'- -cAugUCUCCCGACCUCUGauacaGCUg- -3' miRNA: 3'- cuUugAGGGGGUUGGAGACgc---CGAaa -5' |
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| 13395 | 5' | -55.1 | NC_003409.1 | + | 115503 | 0.68 | 0.768315 |
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Target: 5'- cGGAACUagCCCgGGCCacauUCUGCGGCg-- -3' miRNA: 3'- -CUUUGAg-GGGgUUGG----AGACGCCGaaa -5' |
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| 13395 | 5' | -55.1 | NC_003409.1 | + | 108281 | 0.71 | 0.634456 |
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Target: 5'- ---uUUCUCUgGGCCUCUGCGGCg-- -3' miRNA: 3'- cuuuGAGGGGgUUGGAGACGCCGaaa -5' |
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| 13395 | 5' | -55.1 | NC_003409.1 | + | 61954 | 1.06 | 0.003912 |
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Target: 5'- aGAAACUCCCCCAACCUCUGCGGCUUUg -3' miRNA: 3'- -CUUUGAGGGGGUUGGAGACGCCGAAA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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