Results 1 - 7 of 7 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 134 | 5' | -60.8 | AC_000006.1 | + | 8694 | 0.66 | 0.284531 |
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Target: 5'- cGUGgaGC-GCgCGGGUCGCGGcGAUCu- -3' miRNA: 3'- -CACgaCGaCG-GCUCGGCGCC-CUAGuu -5' |
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| 134 | 5' | -60.8 | AC_000006.1 | + | 13345 | 0.67 | 0.212895 |
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Target: 5'- -cGCUGUcGCCGcGgCGCGGGcgCAGg -3' miRNA: 3'- caCGACGaCGGCuCgGCGCCCuaGUU- -5' |
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| 134 | 5' | -60.8 | AC_000006.1 | + | 3682 | 0.69 | 0.166342 |
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Target: 5'- cUGCgGCUGCCGcGGCUGCGGcGGUg-- -3' miRNA: 3'- cACGaCGACGGC-UCGGCGCC-CUAguu -5' |
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| 134 | 5' | -60.8 | AC_000006.1 | + | 11224 | 0.7 | 0.136283 |
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Target: 5'- -cGCUGCUaaacaucGCCGAGCC-CGaGGGUCGc -3' miRNA: 3'- caCGACGA-------CGGCUCGGcGC-CCUAGUu -5' |
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| 134 | 5' | -60.8 | AC_000006.1 | + | 13179 | 0.74 | 0.068242 |
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Target: 5'- -cGCUGCUGCagccGCCGCGGG-UCAAg -3' miRNA: 3'- caCGACGACGgcu-CGGCGCCCuAGUU- -5' |
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| 134 | 5' | -60.8 | AC_000006.1 | + | 14201 | 0.93 | 0.00225 |
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Target: 5'- gGUGCUGCUGCCGGGCUGCGGGGUgGAc -3' miRNA: 3'- -CACGACGACGGCUCGGCGCCCUAgUU- -5' |
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| 134 | 5' | -60.8 | AC_000006.1 | + | 16118 | 1.05 | 0.000215 |
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Target: 5'- gGUGCUGCUGCCGAGCCGCGGGAUCAAg -3' miRNA: 3'- -CACGACGACGGCUCGGCGCCCUAGUU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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