Results 1 - 15 of 15 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
1340 | 5' | -58.4 | NC_001335.1 | + | 49368 | 0.66 | 0.543152 |
Target: 5'- gGCCAGAGcGAUguGGacugcccgaacugugGUGCCUGCUa- -3' miRNA: 3'- -UGGUCUC-CUGguCCg--------------CAUGGACGAgc -5' |
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1340 | 5' | -58.4 | NC_001335.1 | + | 16690 | 0.66 | 0.542096 |
Target: 5'- -gCAGGGGAgCCAGgaGCGUGCCgaacgaGUUCGc -3' miRNA: 3'- ugGUCUCCU-GGUC--CGCAUGGa-----CGAGC- -5' |
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1340 | 5' | -58.4 | NC_001335.1 | + | 34541 | 0.66 | 0.490266 |
Target: 5'- cACgAGAGGGCUGGGCugcUGCgCUGgUCGa -3' miRNA: 3'- -UGgUCUCCUGGUCCGc--AUG-GACgAGC- -5' |
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1340 | 5' | -58.4 | NC_001335.1 | + | 51384 | 0.66 | 0.490266 |
Target: 5'- uGCCAGAGGACUgaGGGUcgGCggaaGCUCGa -3' miRNA: 3'- -UGGUCUCCUGG--UCCGcaUGga--CGAGC- -5' |
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1340 | 5' | -58.4 | NC_001335.1 | + | 37388 | 0.67 | 0.480172 |
Target: 5'- cCCGGAGGACguGGCcGUcaugaugauCCUGCUg- -3' miRNA: 3'- uGGUCUCCUGguCCG-CAu--------GGACGAgc -5' |
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1340 | 5' | -58.4 | NC_001335.1 | + | 24729 | 0.67 | 0.47018 |
Target: 5'- aACCGGAGGcucguccacGCCAGGaUGUauGCCgcgauccgguagUGCUCGa -3' miRNA: 3'- -UGGUCUCC---------UGGUCC-GCA--UGG------------ACGAGC- -5' |
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1340 | 5' | -58.4 | NC_001335.1 | + | 50844 | 0.67 | 0.460294 |
Target: 5'- aGCCAGGccgacaGGCCGGGCGUcACCgggcaGUUCGa -3' miRNA: 3'- -UGGUCUc-----CUGGUCCGCA-UGGa----CGAGC- -5' |
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1340 | 5' | -58.4 | NC_001335.1 | + | 15096 | 0.69 | 0.351243 |
Target: 5'- gGCCGauGAGGACCGGaGCGUACUUcuuCUUGa -3' miRNA: 3'- -UGGU--CUCCUGGUC-CGCAUGGAc--GAGC- -5' |
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1340 | 5' | -58.4 | NC_001335.1 | + | 13927 | 0.69 | 0.334941 |
Target: 5'- gACCGGAGGAUCGGGgcCGcuacUGCCUGgaCGc -3' miRNA: 3'- -UGGUCUCCUGGUCC--GC----AUGGACgaGC- -5' |
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1340 | 5' | -58.4 | NC_001335.1 | + | 2364 | 0.71 | 0.2823 |
Target: 5'- uACCAGAGGACUGGGagcugaauUACCgGCUCc -3' miRNA: 3'- -UGGUCUCCUGGUCCgc------AUGGaCGAGc -5' |
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1340 | 5' | -58.4 | NC_001335.1 | + | 47765 | 0.72 | 0.236433 |
Target: 5'- aGCCAGcaAGGAUCGcGGCGUugCUGCg-- -3' miRNA: 3'- -UGGUC--UCCUGGU-CCGCAugGACGagc -5' |
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1340 | 5' | -58.4 | NC_001335.1 | + | 12110 | 0.72 | 0.229821 |
Target: 5'- cGCCAGcucgucuGGGGCCAGGuCGgcgGCCUGUccuUCGa -3' miRNA: 3'- -UGGUC-------UCCUGGUCC-GCa--UGGACG---AGC- -5' |
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1340 | 5' | -58.4 | NC_001335.1 | + | 31108 | 0.73 | 0.202242 |
Target: 5'- cACCGagcgcGAGGucaaggacauccGCCAGGCGUACCUGggCGg -3' miRNA: 3'- -UGGU-----CUCC------------UGGUCCGCAUGGACgaGC- -5' |
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1340 | 5' | -58.4 | NC_001335.1 | + | 40640 | 0.73 | 0.188808 |
Target: 5'- cGCCGGAucguguucuucaacgGGAUCAcGGacaaGUACCUGCUCGa -3' miRNA: 3'- -UGGUCU---------------CCUGGU-CCg---CAUGGACGAGC- -5' |
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1340 | 5' | -58.4 | NC_001335.1 | + | 6945 | 1.08 | 0.000563 |
Target: 5'- cACCAGAGGACCAGGCGUACCUGCUCGu -3' miRNA: 3'- -UGGUCUCCUGGUCCGCAUGGACGAGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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