Results 1 - 9 of 9 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 13405 | 5' | -53.2 | NC_003409.1 | + | 15839 | 0.66 | 0.957117 |
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Target: 5'- gGCGCg--GCGAGCccgUGUgggauUCCGUCAUUCa -3' miRNA: 3'- -UGCGaagCGCUUG---ACG-----AGGUAGUGAG- -5' |
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| 13405 | 5' | -53.2 | NC_003409.1 | + | 38677 | 0.66 | 0.957117 |
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Target: 5'- uCGggaUUCGCaGAAUUGCUCCAcgCGCUg -3' miRNA: 3'- uGCg--AAGCG-CUUGACGAGGUa-GUGAg -5' |
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| 13405 | 5' | -53.2 | NC_003409.1 | + | 63506 | 0.66 | 0.950585 |
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Target: 5'- uAUGUgaUCGCGGAcCUGCUUCAgauggagcucauuagUCACUCc -3' miRNA: 3'- -UGCGa-AGCGCUU-GACGAGGU---------------AGUGAG- -5' |
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| 13405 | 5' | -53.2 | NC_003409.1 | + | 10786 | 0.66 | 0.948854 |
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Target: 5'- cACGCUacUCGCGAgucguuucucgcACUGCUgUAcaGCUCg -3' miRNA: 3'- -UGCGA--AGCGCU------------UGACGAgGUagUGAG- -5' |
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| 13405 | 5' | -53.2 | NC_003409.1 | + | 53721 | 0.69 | 0.85492 |
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Target: 5'- uGCGCacaUCaGCGAGCUGgaCCGUC-CUCc -3' miRNA: 3'- -UGCGa--AG-CGCUUGACgaGGUAGuGAG- -5' |
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| 13405 | 5' | -53.2 | NC_003409.1 | + | 67469 | 0.7 | 0.829792 |
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Target: 5'- cACGCUcacgGCGGACgaggGCUCCucgucuguGUCACUCc -3' miRNA: 3'- -UGCGAag--CGCUUGa---CGAGG--------UAGUGAG- -5' |
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| 13405 | 5' | -53.2 | NC_003409.1 | + | 112566 | 0.71 | 0.734933 |
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Target: 5'- cCGUgacggCGCGGACUGCUUCAgcUCACcUCa -3' miRNA: 3'- uGCGaa---GCGCUUGACGAGGU--AGUG-AG- -5' |
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| 13405 | 5' | -53.2 | NC_003409.1 | + | 22198 | 0.73 | 0.634743 |
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Target: 5'- cGCGCUUCGCGAgagguaucuccguguGCUuCUCCAUCAg-- -3' miRNA: 3'- -UGCGAAGCGCU---------------UGAcGAGGUAGUgag -5' |
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| 13405 | 5' | -53.2 | NC_003409.1 | + | 81373 | 1.08 | 0.004694 |
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Target: 5'- uACGCUUCGCGAACUGCUCCAUCACUCc -3' miRNA: 3'- -UGCGAAGCGCUUGACGAGGUAGUGAG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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