Results 21 - 24 of 24 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
13408 | 3' | -54.5 | NC_003409.1 | + | 116553 | 0.73 | 0.63482 |
Target: 5'- -aUGGAGACCCCGauaUGCACcuccaaaacAGUAAUGc -3' miRNA: 3'- ggGCCUCUGGGGUg--ACGUG---------UCAUUAC- -5' |
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13408 | 3' | -54.5 | NC_003409.1 | + | 31077 | 0.73 | 0.624449 |
Target: 5'- aCCGGAGACCacgcauaCACcaUGCGCGGcGGUGg -3' miRNA: 3'- gGGCCUCUGGg------GUG--ACGUGUCaUUAC- -5' |
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13408 | 3' | -54.5 | NC_003409.1 | + | 87882 | 0.75 | 0.502547 |
Target: 5'- gCCGGAGACCCC--UGaCACAGUAccGg -3' miRNA: 3'- gGGCCUCUGGGGugAC-GUGUCAUuaC- -5' |
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13408 | 3' | -54.5 | NC_003409.1 | + | 84223 | 1.11 | 0.002856 |
Target: 5'- gCCCGGAGACCCCACUGCACAGUAAUGa -3' miRNA: 3'- -GGGCCUCUGGGGUGACGUGUCAUUAC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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