Results 1 - 12 of 12 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
1341 | 5' | -62.3 | NC_001335.1 | + | 26648 | 0.66 | 0.369032 |
Target: 5'- cGGUCCccGGCUGAU--CCGCGugGa- -3' miRNA: 3'- aCCAGGa-CCGACUGccGGCGCugCca -5' |
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1341 | 5' | -62.3 | NC_001335.1 | + | 4611 | 0.66 | 0.36076 |
Target: 5'- gGGUCCacgacaUGGCccGCGcUCGCGACGGUc -3' miRNA: 3'- aCCAGG------ACCGacUGCcGGCGCUGCCA- -5' |
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1341 | 5' | -62.3 | NC_001335.1 | + | 38247 | 0.67 | 0.313887 |
Target: 5'- gUGGUcgcCCUGGCgucacugGACGGUagcUGCGAucCGGUa -3' miRNA: 3'- -ACCA---GGACCGa------CUGCCG---GCGCU--GCCA- -5' |
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1341 | 5' | -62.3 | NC_001335.1 | + | 11198 | 0.68 | 0.278463 |
Target: 5'- cGaGUCCUGcGUUcGACccgucucagGGUCGCGGCGGUa -3' miRNA: 3'- aC-CAGGAC-CGA-CUG---------CCGGCGCUGCCA- -5' |
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1341 | 5' | -62.3 | NC_001335.1 | + | 43681 | 0.68 | 0.27111 |
Target: 5'- aGGcUCUGGCcGAgGGCCGCGAgaugagccucgaaCGGg -3' miRNA: 3'- aCCaGGACCGaCUgCCGGCGCU-------------GCCa -5' |
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1341 | 5' | -62.3 | NC_001335.1 | + | 32696 | 0.68 | 0.246297 |
Target: 5'- gUGGaUCCgcgccGGCcaACGGCgGCGGCGGUg -3' miRNA: 3'- -ACC-AGGa----CCGacUGCCGgCGCUGCCA- -5' |
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1341 | 5' | -62.3 | NC_001335.1 | + | 22539 | 0.69 | 0.240246 |
Target: 5'- cUGGUCCUGGUcuggGACGcGCCcgGUGAgGGc -3' miRNA: 3'- -ACCAGGACCGa---CUGC-CGG--CGCUgCCa -5' |
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1341 | 5' | -62.3 | NC_001335.1 | + | 2293 | 0.69 | 0.222834 |
Target: 5'- aGGUCgaGGCUGAacuucGCCGCGA-GGUc -3' miRNA: 3'- aCCAGgaCCGACUgc---CGGCGCUgCCA- -5' |
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1341 | 5' | -62.3 | NC_001335.1 | + | 5943 | 0.69 | 0.217273 |
Target: 5'- gGGUCg-GGCUGGCcuguGCCGUGgACGGUg -3' miRNA: 3'- aCCAGgaCCGACUGc---CGGCGC-UGCCA- -5' |
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1341 | 5' | -62.3 | NC_001335.1 | + | 48364 | 0.71 | 0.167958 |
Target: 5'- gUGGUUC-GGCcGGcCGGCCGCGACaaGGUa -3' miRNA: 3'- -ACCAGGaCCGaCU-GCCGGCGCUG--CCA- -5' |
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1341 | 5' | -62.3 | NC_001335.1 | + | 23738 | 0.81 | 0.02889 |
Target: 5'- aUGGcCCUGGCUGACGGCUGgGGCagaGGUg -3' miRNA: 3'- -ACCaGGACCGACUGCCGGCgCUG---CCA- -5' |
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1341 | 5' | -62.3 | NC_001335.1 | + | 7156 | 1.07 | 0.000289 |
Target: 5'- cUGGUCCUGGCUGACGGCCGCGACGGUg -3' miRNA: 3'- -ACCAGGACCGACUGCCGGCGCUGCCA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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