Results 21 - 23 of 23 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
13411 | 3' | -57.9 | NC_003409.1 | + | 117722 | 0.67 | 0.757441 |
Target: 5'- uGCCCUgguUGACACAauGUGCCGcGCAucaaccAGCgCGa -3' miRNA: 3'- -UGGGG---ACUGUGU--CAUGGC-CGU------UCG-GC- -5' |
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13411 | 3' | -57.9 | NC_003409.1 | + | 21873 | 0.67 | 0.776269 |
Target: 5'- aGCgUCCUGGCAUAG-ACCGGaCAAGUg- -3' miRNA: 3'- -UG-GGGACUGUGUCaUGGCC-GUUCGgc -5' |
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13411 | 3' | -57.9 | NC_003409.1 | + | 44133 | 0.66 | 0.812318 |
Target: 5'- cGCCCCUGACugAGa--CGGau-GUCGa -3' miRNA: 3'- -UGGGGACUGugUCaugGCCguuCGGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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