Results 21 - 28 of 28 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 13411 | 5' | -65.2 | NC_003409.1 | + | 40967 | 0.69 | 0.30207 |
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Target: 5'- cGUGGCUUGcauuaGGCGa-GCCGccGCCCGGg -3' miRNA: 3'- aCGCCGAAC-----CCGCcgCGGCa-CGGGCC- -5' |
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| 13411 | 5' | -65.2 | NC_003409.1 | + | 109849 | 0.7 | 0.289091 |
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Target: 5'- cGCGGCggGaGGCGGUGgC--GCCCGGc -3' miRNA: 3'- aCGCCGaaC-CCGCCGCgGcaCGGGCC- -5' |
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| 13411 | 5' | -65.2 | NC_003409.1 | + | 5022 | 0.71 | 0.247114 |
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Target: 5'- aUGCaacGUauacuGGCaGGCGCCGUGCCCGGu -3' miRNA: 3'- -ACGc--CGaac--CCG-CCGCGGCACGGGCC- -5' |
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| 13411 | 5' | -65.2 | NC_003409.1 | + | 93662 | 0.71 | 0.247114 |
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Target: 5'- gGCGGUggguugugUGGGCGGCGggauguaCGUGCagCGGg -3' miRNA: 3'- aCGCCGa-------ACCCGCCGCg------GCACGg-GCC- -5' |
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| 13411 | 5' | -65.2 | NC_003409.1 | + | 78447 | 0.71 | 0.236085 |
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Target: 5'- aUGCGGCUaccGGGCuGCGCCa-GCCaCGGc -3' miRNA: 3'- -ACGCCGAa--CCCGcCGCGGcaCGG-GCC- -5' |
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| 13411 | 5' | -65.2 | NC_003409.1 | + | 133031 | 0.73 | 0.18656 |
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Target: 5'- uUGCGGUgguGGCagagucgGGCGCgGUGCCUGGg -3' miRNA: 3'- -ACGCCGaacCCG-------CCGCGgCACGGGCC- -5' |
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| 13411 | 5' | -65.2 | NC_003409.1 | + | 132585 | 0.74 | 0.143606 |
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Target: 5'- aUGCuGGCcgagaGGGUGGCGCCcagaGUGCCCGa -3' miRNA: 3'- -ACG-CCGaa---CCCGCCGCGG----CACGGGCc -5' |
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| 13411 | 5' | -65.2 | NC_003409.1 | + | 87924 | 1.08 | 0.000452 |
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Target: 5'- cUGCGGCUUGGGCGGCGCCGUGCCCGGu -3' miRNA: 3'- -ACGCCGAACCCGCCGCGGCACGGGCC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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