Results 1 - 6 of 6 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 13412 | 3' | -58.7 | NC_003409.1 | + | 48796 | 0.66 | 0.731378 |
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Target: 5'- uUGGGCCagGGGUcacaguuuccaGgCCgAGCUAGGUAa -3' miRNA: 3'- -ACCCGGagCUCA-----------CgGGgUCGAUUCAU- -5' |
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| 13412 | 3' | -58.7 | NC_003409.1 | + | 52073 | 0.66 | 0.721438 |
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Target: 5'- cGGaGCCUCGAGcGCCaUguGgUGAGUGg -3' miRNA: 3'- aCC-CGGAGCUCaCGG-GguCgAUUCAU- -5' |
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| 13412 | 3' | -58.7 | NC_003409.1 | + | 36043 | 0.67 | 0.691188 |
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Target: 5'- cGGGCUUUGAGacUGCCCCc-CUuGGUAg -3' miRNA: 3'- aCCCGGAGCUC--ACGGGGucGAuUCAU- -5' |
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| 13412 | 3' | -58.7 | NC_003409.1 | + | 108309 | 0.69 | 0.557874 |
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Target: 5'- cGGGUCUCGAGggacUGUCCUGGCUcGAGc- -3' miRNA: 3'- aCCCGGAGCUC----ACGGGGUCGA-UUCau -5' |
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| 13412 | 3' | -58.7 | NC_003409.1 | + | 109470 | 0.7 | 0.48867 |
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Target: 5'- gUGGGCCcaagaCG-GcGCgCCCAGCUAGGUAg -3' miRNA: 3'- -ACCCGGa----GCuCaCG-GGGUCGAUUCAU- -5' |
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| 13412 | 3' | -58.7 | NC_003409.1 | + | 90236 | 1.06 | 0.001809 |
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Target: 5'- cUGGGCCUCGAGUGCCCCAGCUAAGUAc -3' miRNA: 3'- -ACCCGGAGCUCACGGGGUCGAUUCAU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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