Results 1 - 9 of 9 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 13413 | 3' | -48.8 | NC_003409.1 | + | 44541 | 0.66 | 0.997626 |
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Target: 5'- gGGUGgcGCGGUAcgAUCCUCUcgcuccaggacaGGGGu -3' miRNA: 3'- aCUACaaCGCUAUugUAGGAGA------------CCCU- -5' |
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| 13413 | 3' | -48.8 | NC_003409.1 | + | 88236 | 0.66 | 0.997495 |
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Target: 5'- aUGAgg--GCGcaucuaccucagguAUGGgGUCCUCUGGGAc -3' miRNA: 3'- -ACUacaaCGC--------------UAUUgUAGGAGACCCU- -5' |
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| 13413 | 3' | -48.8 | NC_003409.1 | + | 75279 | 0.67 | 0.996032 |
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Target: 5'- gGAagcUGUUGCGAaaugUGugGUCCUCUuGGuGGu -3' miRNA: 3'- aCU---ACAACGCU----AUugUAGGAGA-CC-CU- -5' |
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| 13413 | 3' | -48.8 | NC_003409.1 | + | 110733 | 0.67 | 0.995966 |
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Target: 5'- -cGUGgcgGCGAccucguggauuucUGACAUUgUCUGGGAa -3' miRNA: 3'- acUACaa-CGCU-------------AUUGUAGgAGACCCU- -5' |
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| 13413 | 3' | -48.8 | NC_003409.1 | + | 52328 | 0.67 | 0.993651 |
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Target: 5'- cGGUG--GCGggGGCAcgggcUCUUCUGGGAa -3' miRNA: 3'- aCUACaaCGCuaUUGU-----AGGAGACCCU- -5' |
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| 13413 | 3' | -48.8 | NC_003409.1 | + | 133897 | 0.68 | 0.992641 |
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Target: 5'- aUGAUGUcauuaugcaggcUGUuAUGAUAUCC-CUGGGGg -3' miRNA: 3'- -ACUACA------------ACGcUAUUGUAGGaGACCCU- -5' |
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| 13413 | 3' | -48.8 | NC_003409.1 | + | 22628 | 0.68 | 0.991507 |
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Target: 5'- gGGUGUUGCGcgcACAuuuggggcugcUCUUCUGGGu -3' miRNA: 3'- aCUACAACGCuauUGU-----------AGGAGACCCu -5' |
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| 13413 | 3' | -48.8 | NC_003409.1 | + | 68293 | 0.73 | 0.902619 |
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Target: 5'- cGAUGgcguguccucgGCGAaGACGUCgUCUGGGGc -3' miRNA: 3'- aCUACaa---------CGCUaUUGUAGgAGACCCU- -5' |
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| 13413 | 3' | -48.8 | NC_003409.1 | + | 90349 | 1.1 | 0.010335 |
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Target: 5'- gUGAUGUUGCGAUAACAUCCUCUGGGAu -3' miRNA: 3'- -ACUACAACGCUAUUGUAGGAGACCCU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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