Results 1 - 7 of 7 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 13414 | 3' | -57.1 | NC_003409.1 | + | 25815 | 0.66 | 0.867614 |
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Target: 5'- cCGucacGUUCUUUGUCUCCAGAGgCGa -3' miRNA: 3'- aGCucc-CAGGAAGCAGAGGUCUCgGU- -5' |
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| 13414 | 3' | -57.1 | NC_003409.1 | + | 3772 | 0.66 | 0.836271 |
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Target: 5'- cUGAGGGcaugCCUcUGggCUCCAGAGCg- -3' miRNA: 3'- aGCUCCCa---GGAaGCa-GAGGUCUCGgu -5' |
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| 13414 | 3' | -57.1 | NC_003409.1 | + | 43714 | 0.68 | 0.755743 |
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Target: 5'- aCGcu-GUCCgUCGUCUCCGGAcGCCGg -3' miRNA: 3'- aGCuccCAGGaAGCAGAGGUCU-CGGU- -5' |
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| 13414 | 3' | -57.1 | NC_003409.1 | + | 64753 | 0.69 | 0.720707 |
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Target: 5'- gUGGGGGUcuuacagaaggucaaCCUUCGaCUCCuGGGCCc -3' miRNA: 3'- aGCUCCCA---------------GGAAGCaGAGGuCUCGGu -5' |
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| 13414 | 3' | -57.1 | NC_003409.1 | + | 31341 | 0.71 | 0.574819 |
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Target: 5'- -aGAGGuGUCCUUCGUCgUCCucuGcGCCGc -3' miRNA: 3'- agCUCC-CAGGAAGCAG-AGGu--CuCGGU- -5' |
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| 13414 | 3' | -57.1 | NC_003409.1 | + | 90639 | 0.82 | 0.138268 |
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Target: 5'- gUCGAuGGUCCcUCGUCUCCAGAGUCc -3' miRNA: 3'- -AGCUcCCAGGaAGCAGAGGUCUCGGu -5' |
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| 13414 | 3' | -57.1 | NC_003409.1 | + | 90558 | 1.09 | 0.002069 |
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Target: 5'- gUCGAGGGUCCUUCGUCUCCAGAGCCAg -3' miRNA: 3'- -AGCUCCCAGGAAGCAGAGGUCUCGGU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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