Results 21 - 27 of 27 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
13414 | 5' | -50.3 | NC_003409.1 | + | 68513 | 0.69 | 0.939903 |
Target: 5'- uUUGGCAggccgCG-CGGCUauGCUGGACCCa -3' miRNA: 3'- uAACCGUa----GUaGUUGAc-CGACUUGGG- -5' |
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13414 | 5' | -50.3 | NC_003409.1 | + | 32296 | 0.69 | 0.939903 |
Target: 5'- -cUGGCAUCca-GGCUGGC-GGACaCCu -3' miRNA: 3'- uaACCGUAGuagUUGACCGaCUUG-GG- -5' |
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13414 | 5' | -50.3 | NC_003409.1 | + | 31030 | 0.71 | 0.898306 |
Target: 5'- -gUGGC-UCA-CAugUGGCUGAGCa- -3' miRNA: 3'- uaACCGuAGUaGUugACCGACUUGgg -5' |
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13414 | 5' | -50.3 | NC_003409.1 | + | 65041 | 0.72 | 0.863977 |
Target: 5'- uUUGGCAUCcgauGUCAugUugaggaugaacccggGGCUGGGCUCg -3' miRNA: 3'- uAACCGUAG----UAGUugA---------------CCGACUUGGG- -5' |
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13414 | 5' | -50.3 | NC_003409.1 | + | 132664 | 0.75 | 0.696446 |
Target: 5'- --cGGCcUCAUCGcccaacagGCUGGCgugGGACCCc -3' miRNA: 3'- uaaCCGuAGUAGU--------UGACCGa--CUUGGG- -5' |
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13414 | 5' | -50.3 | NC_003409.1 | + | 57074 | 0.8 | 0.424439 |
Target: 5'- --gGGCGUcCGUCucuGgUGGCUGAACCCa -3' miRNA: 3'- uaaCCGUA-GUAGu--UgACCGACUUGGG- -5' |
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13414 | 5' | -50.3 | NC_003409.1 | + | 90595 | 1.1 | 0.006702 |
Target: 5'- cAUUGGCAUCAUCAACUGGCUGAACCCc -3' miRNA: 3'- -UAACCGUAGUAGUUGACCGACUUGGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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