Results 1 - 9 of 9 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 13417 | 3' | -53.8 | NC_003409.1 | + | 52580 | 0.66 | 0.950031 |
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Target: 5'- --gGCGUGGCGGccaAGGgGCgGGGACGu -3' miRNA: 3'- cuaCGCACCGCCc--UUCaUGaCUCUGU- -5' |
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| 13417 | 3' | -53.8 | NC_003409.1 | + | 75471 | 0.66 | 0.950031 |
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Target: 5'- --cGCGgGGaGGGggGUguuauaggggcgGCUGGGACGg -3' miRNA: 3'- cuaCGCaCCgCCCuuCA------------UGACUCUGU- -5' |
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| 13417 | 3' | -53.8 | NC_003409.1 | + | 100085 | 0.66 | 0.945623 |
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Target: 5'- cGUGCGggccaaugccaUGGCGGGgcG-GCUGuGGCAc -3' miRNA: 3'- cUACGC-----------ACCGCCCuuCaUGACuCUGU- -5' |
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| 13417 | 3' | -53.8 | NC_003409.1 | + | 50192 | 0.66 | 0.945623 |
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Target: 5'- --cGCGUGGUGGGugacgcAGcGCUGAcACAg -3' miRNA: 3'- cuaCGCACCGCCCu-----UCaUGACUcUGU- -5' |
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| 13417 | 3' | -53.8 | NC_003409.1 | + | 67405 | 0.67 | 0.919825 |
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Target: 5'- --aGUG-GGC-GGAAGUACUGGGAg- -3' miRNA: 3'- cuaCGCaCCGcCCUUCAUGACUCUgu -5' |
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| 13417 | 3' | -53.8 | NC_003409.1 | + | 14244 | 0.7 | 0.788025 |
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Target: 5'- --cGCGUGGCGGuGGAccuGUACUucGACAa -3' miRNA: 3'- cuaCGCACCGCC-CUU---CAUGAcuCUGU- -5' |
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| 13417 | 3' | -53.8 | NC_003409.1 | + | 54408 | 0.7 | 0.778541 |
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Target: 5'- --gGCGUGGCGGGAuGGUGCcGuGuGCAg -3' miRNA: 3'- cuaCGCACCGCCCU-UCAUGaCuC-UGU- -5' |
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| 13417 | 3' | -53.8 | NC_003409.1 | + | 92800 | 0.99 | 0.016712 |
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Target: 5'- gGAUGCGUGGCGGG-AGUACUGAGACAc -3' miRNA: 3'- -CUACGCACCGCCCuUCAUGACUCUGU- -5' |
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| 13417 | 3' | -53.8 | NC_003409.1 | + | 92626 | 1.08 | 0.00438 |
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Target: 5'- gGAUGCGUGGCGGGAAGUACUGAGACAc -3' miRNA: 3'- -CUACGCACCGCCCUUCAUGACUCUGU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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