Results 1 - 9 of 9 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 13419 | 3' | -48.7 | NC_003409.1 | + | 13234 | 0.66 | 0.998727 |
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Target: 5'- aCGAGUACGgggUCGuCAuagaaucCCGGAgaggGGCUUa -3' miRNA: 3'- aGCUCAUGUa--AGC-GU-------GGCUUa---UCGAG- -5' |
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| 13419 | 3' | -48.7 | NC_003409.1 | + | 10776 | 0.66 | 0.998168 |
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Target: 5'- gCGAGU-CGUuucUCGCACUGcuGUacAGCUCg -3' miRNA: 3'- aGCUCAuGUA---AGCGUGGCu-UA--UCGAG- -5' |
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| 13419 | 3' | -48.7 | NC_003409.1 | + | 117962 | 0.66 | 0.998168 |
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Target: 5'- aCGAGUugcaACGggcgCGCACUGAAgcUAGCg- -3' miRNA: 3'- aGCUCA----UGUaa--GCGUGGCUU--AUCGag -5' |
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| 13419 | 3' | -48.7 | NC_003409.1 | + | 78409 | 0.67 | 0.996303 |
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Target: 5'- cUCGAGUGCAUuuUCGCGagGAuacaaAUAGCg- -3' miRNA: 3'- -AGCUCAUGUA--AGCGUggCU-----UAUCGag -5' |
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| 13419 | 3' | -48.7 | NC_003409.1 | + | 20195 | 0.67 | 0.994901 |
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Target: 5'- -gGAGUGCGcgUCaGCugcaGCCGuAGUGGCUCu -3' miRNA: 3'- agCUCAUGUa-AG-CG----UGGC-UUAUCGAG- -5' |
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| 13419 | 3' | -48.7 | NC_003409.1 | + | 50256 | 0.67 | 0.994055 |
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Target: 5'- -gGAGgggGCAUUCGaCACCGAAcuaaugGGCg- -3' miRNA: 3'- agCUCa--UGUAAGC-GUGGCUUa-----UCGag -5' |
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| 13419 | 3' | -48.7 | NC_003409.1 | + | 14218 | 0.68 | 0.993098 |
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Target: 5'- cCGAGUACGUUaagcaGCACgGAcugcgcGUGGCg- -3' miRNA: 3'- aGCUCAUGUAAg----CGUGgCU------UAUCGag -5' |
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| 13419 | 3' | -48.7 | NC_003409.1 | + | 37954 | 0.69 | 0.984512 |
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Target: 5'- aUCGAGgcCAggCGCGCCugcgcAGCUCa -3' miRNA: 3'- -AGCUCauGUaaGCGUGGcuua-UCGAG- -5' |
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| 13419 | 3' | -48.7 | NC_003409.1 | + | 96329 | 1.12 | 0.008146 |
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Target: 5'- gUCGAGUACAUUCGCACCGAAUAGCUCa -3' miRNA: 3'- -AGCUCAUGUAAGCGUGGCUUAUCGAG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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