Results 1 - 20 of 26 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
1342 | 3' | -56.3 | NC_001335.1 | + | 43735 | 0.66 | 0.631234 |
Target: 5'- -cGGCGugguuGCcGCAGGgcaccgcguuucacaCCGUCGCUGAg -3' miRNA: 3'- uaCUGCu----UGuCGUCC---------------GGCAGCGACUg -5' |
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1342 | 3' | -56.3 | NC_001335.1 | + | 22971 | 0.66 | 0.62678 |
Target: 5'- gGUGAUGccGACGGUcggAGcGuuGUCGUUGACg -3' miRNA: 3'- -UACUGC--UUGUCG---UC-CggCAGCGACUG- -5' |
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1342 | 3' | -56.3 | NC_001335.1 | + | 1155 | 0.66 | 0.62678 |
Target: 5'- -gGAuCGGGCGGCguAGGCggCGUCGgaGGCg -3' miRNA: 3'- uaCU-GCUUGUCG--UCCG--GCAGCgaCUG- -5' |
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1342 | 3' | -56.3 | NC_001335.1 | + | 29080 | 0.66 | 0.62678 |
Target: 5'- gGUGugGAGCGGCuGaGCaagaaGCUGGCg -3' miRNA: 3'- -UACugCUUGUCGuC-CGgcag-CGACUG- -5' |
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1342 | 3' | -56.3 | NC_001335.1 | + | 4688 | 0.66 | 0.615648 |
Target: 5'- --cGCGAGC-GCGGGCCauGUCGUgGACc -3' miRNA: 3'- uacUGCUUGuCGUCCGG--CAGCGaCUG- -5' |
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1342 | 3' | -56.3 | NC_001335.1 | + | 34330 | 0.66 | 0.615648 |
Target: 5'- --aGCGAacgGCAGCGGGCC---GCUGGCc -3' miRNA: 3'- uacUGCU---UGUCGUCCGGcagCGACUG- -5' |
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1342 | 3' | -56.3 | NC_001335.1 | + | 26675 | 0.66 | 0.612311 |
Target: 5'- -cGACGGcCGGCucaAGGCCGUUcggcucgguccccgGCUGAUc -3' miRNA: 3'- uaCUGCUuGUCG---UCCGGCAG--------------CGACUG- -5' |
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1342 | 3' | -56.3 | NC_001335.1 | + | 45201 | 0.66 | 0.59233 |
Target: 5'- cUGGCGGGCGGUcuccuucAGGUCGgacuUCGCcGACa -3' miRNA: 3'- uACUGCUUGUCG-------UCCGGC----AGCGaCUG- -5' |
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1342 | 3' | -56.3 | NC_001335.1 | + | 42285 | 0.66 | 0.582378 |
Target: 5'- cGUGACGAcGCGGCAGaggaGCUGcacgCGCUGGu -3' miRNA: 3'- -UACUGCU-UGUCGUC----CGGCa---GCGACUg -5' |
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1342 | 3' | -56.3 | NC_001335.1 | + | 37260 | 0.67 | 0.560394 |
Target: 5'- -gGACGAGCGGUaucccaagGGGCC-UCGCcaguggGACa -3' miRNA: 3'- uaCUGCUUGUCG--------UCCGGcAGCGa-----CUG- -5' |
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1342 | 3' | -56.3 | NC_001335.1 | + | 42590 | 0.67 | 0.560394 |
Target: 5'- cAUGACGAACu--GGGauccaaaccacCCGUCGCUGAg -3' miRNA: 3'- -UACUGCUUGucgUCC-----------GGCAGCGACUg -5' |
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1342 | 3' | -56.3 | NC_001335.1 | + | 4162 | 0.67 | 0.517194 |
Target: 5'- -aGAUGGugGGCcagacggccuucGGGCCGUCccCUGACg -3' miRNA: 3'- uaCUGCUugUCG------------UCCGGCAGc-GACUG- -5' |
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1342 | 3' | -56.3 | NC_001335.1 | + | 3110 | 0.68 | 0.475386 |
Target: 5'- -aGGCGAGCGGCGGGa----GCUGAUg -3' miRNA: 3'- uaCUGCUUGUCGUCCggcagCGACUG- -5' |
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1342 | 3' | -56.3 | NC_001335.1 | + | 21262 | 0.69 | 0.435333 |
Target: 5'- uUGugGAaguGCGGCuuucGGCaCGUCGaCUGGCu -3' miRNA: 3'- uACugCU---UGUCGu---CCG-GCAGC-GACUG- -5' |
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1342 | 3' | -56.3 | NC_001335.1 | + | 50181 | 0.69 | 0.435333 |
Target: 5'- uUGGC--ACAGaAGGCCGUCGCguccgGGCa -3' miRNA: 3'- uACUGcuUGUCgUCCGGCAGCGa----CUG- -5' |
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1342 | 3' | -56.3 | NC_001335.1 | + | 32102 | 0.69 | 0.432407 |
Target: 5'- -cGGCGAcguucuacaucgagGcCAGCAGGUCGgUGCUGACc -3' miRNA: 3'- uaCUGCU--------------U-GUCGUCCGGCaGCGACUG- -5' |
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1342 | 3' | -56.3 | NC_001335.1 | + | 41340 | 0.69 | 0.426592 |
Target: 5'- -cGaACGAGCuacGCGGGCUGUCGCgagcagaacaucgagGACg -3' miRNA: 3'- uaC-UGCUUGu--CGUCCGGCAGCGa--------------CUG- -5' |
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1342 | 3' | -56.3 | NC_001335.1 | + | 19432 | 0.7 | 0.36147 |
Target: 5'- gAUGGCGAcuuccGCcuGCGcGGCCugGUCGCUGGCg -3' miRNA: 3'- -UACUGCU-----UGu-CGU-CCGG--CAGCGACUG- -5' |
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1342 | 3' | -56.3 | NC_001335.1 | + | 22232 | 0.7 | 0.36147 |
Target: 5'- -cGGCGAacuucaggaACAGCGGGCCGUC-UUGGa -3' miRNA: 3'- uaCUGCU---------UGUCGUCCGGCAGcGACUg -5' |
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1342 | 3' | -56.3 | NC_001335.1 | + | 42159 | 0.71 | 0.336111 |
Target: 5'- cAUGGCuuccuuucaGAACGGCGGGCCGUacaGCUcGAUc -3' miRNA: 3'- -UACUG---------CUUGUCGUCCGGCAg--CGA-CUG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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