Results 1 - 11 of 11 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
1342 | 5' | -56.9 | NC_001335.1 | + | 8075 | 0.66 | 0.644679 |
Target: 5'- aCGuCG-GCGCGGaacgCCAccacGUCGUagUGGGCg -3' miRNA: 3'- -GCuGCuCGCGCCa---GGU----CAGCA--ACCUG- -5' |
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1342 | 5' | -56.9 | NC_001335.1 | + | 14821 | 0.66 | 0.633839 |
Target: 5'- gGACGGGCaGCGcagCCAGgucaUCGaUGGGCa -3' miRNA: 3'- gCUGCUCG-CGCca-GGUC----AGCaACCUG- -5' |
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1342 | 5' | -56.9 | NC_001335.1 | + | 22559 | 0.66 | 0.622999 |
Target: 5'- uCGGCGggcucAGCGaCGGUCUGGUCcugGUcugGGACg -3' miRNA: 3'- -GCUGC-----UCGC-GCCAGGUCAG---CAa--CCUG- -5' |
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1342 | 5' | -56.9 | NC_001335.1 | + | 49019 | 0.66 | 0.601349 |
Target: 5'- uGACGAGCcggucgauGCGGUCC--UCG-UGGAa -3' miRNA: 3'- gCUGCUCG--------CGCCAGGucAGCaACCUg -5' |
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1342 | 5' | -56.9 | NC_001335.1 | + | 24387 | 0.66 | 0.601349 |
Target: 5'- cCGAC-AGCGCGGa--GGUCG-UGGAUg -3' miRNA: 3'- -GCUGcUCGCGCCaggUCAGCaACCUG- -5' |
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1342 | 5' | -56.9 | NC_001335.1 | + | 42113 | 0.67 | 0.590559 |
Target: 5'- uGGCGAagGCGUGGgacUCGGUCGUcggcaaGGACg -3' miRNA: 3'- gCUGCU--CGCGCCa--GGUCAGCAa-----CCUG- -5' |
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1342 | 5' | -56.9 | NC_001335.1 | + | 32868 | 0.67 | 0.579802 |
Target: 5'- cCGACG-GCGCGGUCuCAGgcucaGgagccgGGGCc -3' miRNA: 3'- -GCUGCuCGCGCCAG-GUCag---Caa----CCUG- -5' |
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1342 | 5' | -56.9 | NC_001335.1 | + | 26631 | 0.68 | 0.537272 |
Target: 5'- gCGugGAGCGCGaGUC-GGUCGa-GGAg -3' miRNA: 3'- -GCugCUCGCGC-CAGgUCAGCaaCCUg -5' |
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1342 | 5' | -56.9 | NC_001335.1 | + | 15773 | 0.68 | 0.510207 |
Target: 5'- gCGGCGAacgaacccggcuucgGUGUGGUCUucucuaGGUCGUcgGGACg -3' miRNA: 3'- -GCUGCU---------------CGCGCCAGG------UCAGCAa-CCUG- -5' |
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1342 | 5' | -56.9 | NC_001335.1 | + | 4688 | 0.77 | 0.141326 |
Target: 5'- -cGCGAGCGCGGgCCAuGUCG-UGGACc -3' miRNA: 3'- gcUGCUCGCGCCaGGU-CAGCaACCUG- -5' |
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1342 | 5' | -56.9 | NC_001335.1 | + | 7931 | 1.09 | 0.000693 |
Target: 5'- cCGACGAGCGCGGUCCAGUCGUUGGACu -3' miRNA: 3'- -GCUGCUCGCGCCAGGUCAGCAACCUG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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