Results 21 - 29 of 29 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 13422 | 5' | -50.1 | NC_003409.1 | + | 72870 | 0.7 | 0.929055 |
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Target: 5'- aUGCAGCGGGGUgagccugccuccAGCCAu--AUGUAa -3' miRNA: 3'- cGCGUCGCUCCA------------UCGGUuuuUGCAUg -5' |
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| 13422 | 5' | -50.1 | NC_003409.1 | + | 57757 | 0.71 | 0.923418 |
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Target: 5'- uGCGguGCGAGGaGGUggUGAAAACGuUGCu -3' miRNA: 3'- -CGCguCGCUCCaUCG--GUUUUUGC-AUG- -5' |
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| 13422 | 5' | -50.1 | NC_003409.1 | + | 52157 | 0.71 | 0.911349 |
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Target: 5'- uCGCAcGgGGGGUGGCCuguGGCGUGu -3' miRNA: 3'- cGCGU-CgCUCCAUCGGuuuUUGCAUg -5' |
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| 13422 | 5' | -50.1 | NC_003409.1 | + | 43537 | 0.71 | 0.911348 |
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Target: 5'- uGgGCAGC-AGGUGGCCGgcguccugGAGACG-ACg -3' miRNA: 3'- -CgCGUCGcUCCAUCGGU--------UUUUGCaUG- -5' |
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| 13422 | 5' | -50.1 | NC_003409.1 | + | 45891 | 0.72 | 0.891282 |
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Target: 5'- gGCGCc-CGGGGUGGCCugacAAGACGcgGCa -3' miRNA: 3'- -CGCGucGCUCCAUCGGu---UUUUGCa-UG- -5' |
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| 13422 | 5' | -50.1 | NC_003409.1 | + | 131259 | 0.73 | 0.83589 |
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Target: 5'- gGCGCGGgGAGGUggGGCaCGAAAACagGCg -3' miRNA: 3'- -CGCGUCgCUCCA--UCG-GUUUUUGcaUG- -5' |
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| 13422 | 5' | -50.1 | NC_003409.1 | + | 119802 | 0.73 | 0.83589 |
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Target: 5'- gGCGcCGGCGuGGgcGCCGcgcGCGUGCu -3' miRNA: 3'- -CGC-GUCGCuCCauCGGUuuuUGCAUG- -5' |
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| 13422 | 5' | -50.1 | NC_003409.1 | + | 84380 | 0.75 | 0.750431 |
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Target: 5'- uGUGCAGUGGGGUcuccgGGCCAGGuauggcuacccAAUGUGCc -3' miRNA: 3'- -CGCGUCGCUCCA-----UCGGUUU-----------UUGCAUG- -5' |
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| 13422 | 5' | -50.1 | NC_003409.1 | + | 99515 | 1.12 | 0.005585 |
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Target: 5'- uGCGCAGCGAGGUAGCCAAAAACGUACu -3' miRNA: 3'- -CGCGUCGCUCCAUCGGUUUUUGCAUG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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