Results 1 - 6 of 6 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 13425 | 5' | -53.7 | NC_003409.1 | + | 37973 | 0.66 | 0.949402 |
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Target: 5'- -gCGCAGCUCAagagGCGGcGCCGgacauauUCUUgGu -3' miRNA: 3'- gaGCGUUGAGUa---CGCC-CGGU-------AGAAgC- -5' |
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| 13425 | 5' | -53.7 | NC_003409.1 | + | 133313 | 0.66 | 0.940784 |
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Target: 5'- aUCGCGacaGCUCcgacaAUGCGGuGCgCAUCUUgGa -3' miRNA: 3'- gAGCGU---UGAG-----UACGCC-CG-GUAGAAgC- -5' |
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| 13425 | 5' | -53.7 | NC_003409.1 | + | 65986 | 0.67 | 0.93589 |
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Target: 5'- -cUGCAuACUCGaGCGGGuCCAgaaucuuacUCUUCGg -3' miRNA: 3'- gaGCGU-UGAGUaCGCCC-GGU---------AGAAGC- -5' |
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| 13425 | 5' | -53.7 | NC_003409.1 | + | 117814 | 0.68 | 0.887058 |
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Target: 5'- gUUGCAACUCGUGUccugaaugcuacgGGGCCAcgCUggccacUCGg -3' miRNA: 3'- gAGCGUUGAGUACG-------------CCCGGUa-GA------AGC- -5' |
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| 13425 | 5' | -53.7 | NC_003409.1 | + | 120687 | 0.71 | 0.769514 |
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Target: 5'- gCUCGCGuaugcCUCAUGCGaGGUUcgCUUUGg -3' miRNA: 3'- -GAGCGUu----GAGUACGC-CCGGuaGAAGC- -5' |
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| 13425 | 5' | -53.7 | NC_003409.1 | + | 102502 | 1.1 | 0.003687 |
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Target: 5'- cCUCGCAACUCAUGCGGGCCAUCUUCGa -3' miRNA: 3'- -GAGCGUUGAGUACGCCCGGUAGAAGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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