Results 21 - 26 of 26 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 1343 | 5' | -58.1 | NC_001335.1 | + | 39623 | 0.69 | 0.354715 |
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Target: 5'- --uGC-CAGCCCGCUUGCCGU-AGCGu -3' miRNA: 3'- uggUGaGUCGGGUGAGUGGCAgUCGC- -5' |
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| 1343 | 5' | -58.1 | NC_001335.1 | + | 32953 | 0.7 | 0.338262 |
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Target: 5'- gGCCccgGCUCcugAGCCUgagACcgCGCCGUCGGCGa -3' miRNA: 3'- -UGG---UGAG---UCGGG---UGa-GUGGCAGUCGC- -5' |
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| 1343 | 5' | -58.1 | NC_001335.1 | + | 7055 | 0.7 | 0.307045 |
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Target: 5'- aGCCGCguUCAGCCgCGuggaUCACCGUC-GCGg -3' miRNA: 3'- -UGGUG--AGUCGG-GUg---AGUGGCAGuCGC- -5' |
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| 1343 | 5' | -58.1 | NC_001335.1 | + | 27816 | 0.71 | 0.285112 |
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Target: 5'- aACCAC---GCCCAUUCGCCGUC-GUGg -3' miRNA: 3'- -UGGUGaguCGGGUGAGUGGCAGuCGC- -5' |
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| 1343 | 5' | -58.1 | NC_001335.1 | + | 37982 | 0.73 | 0.198907 |
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Target: 5'- uCCGCUgAGCCC---CACCGUCGGCa -3' miRNA: 3'- uGGUGAgUCGGGugaGUGGCAGUCGc -5' |
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| 1343 | 5' | -58.1 | NC_001335.1 | + | 9048 | 1.07 | 0.000632 |
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Target: 5'- aACCACUCAGCCCACUCACCGUCAGCGc -3' miRNA: 3'- -UGGUGAGUCGGGUGAGUGGCAGUCGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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