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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 13430 | 3' | -47.2 | NC_003409.1 | + | 32024 | 0.66 | 0.999657 |
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Target: 5'- cGCCCACaUCCUCCAGc---------- -3' miRNA: 3'- -UGGGUGcAGGAGGUCaaaaaguaaaa -5' |
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| 13430 | 3' | -47.2 | NC_003409.1 | + | 39540 | 0.66 | 0.999657 |
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Target: 5'- -aCCGCGUCCUCCAcccga-CGUUUg -3' miRNA: 3'- ugGGUGCAGGAGGUcaaaaaGUAAAa -5' |
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| 13430 | 3' | -47.2 | NC_003409.1 | + | 112085 | 0.66 | 0.999657 |
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Target: 5'- cACCC-UGUCCUCUGGUccccaUUCAUUg- -3' miRNA: 3'- -UGGGuGCAGGAGGUCAaa---AAGUAAaa -5' |
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| 13430 | 3' | -47.2 | NC_003409.1 | + | 93162 | 0.66 | 0.999565 |
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Target: 5'- gGCCC-CGUCC-CCAGUcaugUCAg--- -3' miRNA: 3'- -UGGGuGCAGGaGGUCAaaa-AGUaaaa -5' |
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| 13430 | 3' | -47.2 | NC_003409.1 | + | 131343 | 0.66 | 0.999373 |
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Target: 5'- gACCCAgguccuuccugugcaUGUCCUCCAGUgggugCAg--- -3' miRNA: 3'- -UGGGU---------------GCAGGAGGUCAaaaa-GUaaaa -5' |
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| 13430 | 3' | -47.2 | NC_003409.1 | + | 100033 | 0.66 | 0.999315 |
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Target: 5'- gACCCGCGUCgCUCUGGUgggaCAg--- -3' miRNA: 3'- -UGGGUGCAG-GAGGUCAaaaaGUaaaa -5' |
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| 13430 | 3' | -47.2 | NC_003409.1 | + | 25633 | 0.66 | 0.999149 |
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Target: 5'- cAUCCGUGUCCUCUAGU-UUUCGg--- -3' miRNA: 3'- -UGGGUGCAGGAGGUCAaAAAGUaaaa -5' |
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| 13430 | 3' | -47.2 | NC_003409.1 | + | 65147 | 0.66 | 0.999149 |
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Target: 5'- gGCCUuCGUCUUCCAGUggaUCGa--- -3' miRNA: 3'- -UGGGuGCAGGAGGUCAaaaAGUaaaa -5' |
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| 13430 | 3' | -47.2 | NC_003409.1 | + | 11843 | 0.77 | 0.779886 |
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Target: 5'- gACCCACGUCCUCCAGc---------- -3' miRNA: 3'- -UGGGUGCAGGAGGUCaaaaaguaaaa -5' |
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| 13430 | 3' | -47.2 | NC_003409.1 | + | 105628 | 1.06 | 0.023106 |
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Target: 5'- cACCCACGUCCUCCAGUUUUUCAUUUUg -3' miRNA: 3'- -UGGGUGCAGGAGGUCAAAAAGUAAAA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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