Results 21 - 27 of 27 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 13430 | 5' | -51.5 | NC_003409.1 | + | 113698 | 0.71 | 0.863963 |
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Target: 5'- gAGGGCgagaaUGGCGUGCCAGUGGAGGAa- -3' miRNA: 3'- -UUUUGa----GCCGUAUGGUUGCCUCCUgc -5' |
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| 13430 | 5' | -51.5 | NC_003409.1 | + | 87171 | 0.71 | 0.847766 |
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Target: 5'- -----gUGGUGcGCCGGCGGAGGGCGu -3' miRNA: 3'- uuuugaGCCGUaUGGUUGCCUCCUGC- -5' |
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| 13430 | 5' | -51.5 | NC_003409.1 | + | 56002 | 0.72 | 0.830718 |
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Target: 5'- cAAugUCGGCGacCCGugGGAGGuCGc -3' miRNA: 3'- uUUugAGCCGUauGGUugCCUCCuGC- -5' |
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| 13430 | 5' | -51.5 | NC_003409.1 | + | 40462 | 0.72 | 0.821002 |
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Target: 5'- cGGAACUCGuaacauaGCuuagACCAGCGGAcGGACGc -3' miRNA: 3'- -UUUUGAGC-------CGua--UGGUUGCCU-CCUGC- -5' |
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| 13430 | 5' | -51.5 | NC_003409.1 | + | 101726 | 0.73 | 0.775152 |
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Target: 5'- ---cCUCuGGCAUACCcuCGGAGGAgGu -3' miRNA: 3'- uuuuGAG-CCGUAUGGuuGCCUCCUgC- -5' |
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| 13430 | 5' | -51.5 | NC_003409.1 | + | 30954 | 0.73 | 0.775152 |
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Target: 5'- --uGCUCuGCGUGCUggaGGCGGGGGugGg -3' miRNA: 3'- uuuUGAGcCGUAUGG---UUGCCUCCugC- -5' |
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| 13430 | 5' | -51.5 | NC_003409.1 | + | 105663 | 1.06 | 0.010087 |
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Target: 5'- aAAAACUCGGCAUACCAACGGAGGACGc -3' miRNA: 3'- -UUUUGAGCCGUAUGGUUGCCUCCUGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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