Results 1 - 9 of 9 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 13432 | 3' | -49.1 | NC_003409.1 | + | 15218 | 0.66 | 0.998113 |
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Target: 5'- uGCAaGAAGgcCCGUGUUCGuuuCGuCUACa -3' miRNA: 3'- -UGUaCUUCa-GGCACAAGUu--GCuGGUG- -5' |
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| 13432 | 3' | -49.1 | NC_003409.1 | + | 132299 | 0.66 | 0.997737 |
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Target: 5'- gGCGUGGAGUCCa-----GAgGACCACc -3' miRNA: 3'- -UGUACUUCAGGcacaagUUgCUGGUG- -5' |
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| 13432 | 3' | -49.1 | NC_003409.1 | + | 12571 | 0.66 | 0.997737 |
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Target: 5'- ----uGGGUCCGUGUUUGAgGuCCACc -3' miRNA: 3'- uguacUUCAGGCACAAGUUgCuGGUG- -5' |
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| 13432 | 3' | -49.1 | NC_003409.1 | + | 131172 | 0.67 | 0.993939 |
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Target: 5'- cCGUGGccaccucaaAGcCCGUGUUCugcaacGCGGCCAUg -3' miRNA: 3'- uGUACU---------UCaGGCACAAGu-----UGCUGGUG- -5' |
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| 13432 | 3' | -49.1 | NC_003409.1 | + | 67778 | 0.68 | 0.986155 |
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Target: 5'- ----aGAGUCCGaUGUcaggUCGACGAUCGCg -3' miRNA: 3'- uguacUUCAGGC-ACA----AGUUGCUGGUG- -5' |
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| 13432 | 3' | -49.1 | NC_003409.1 | + | 108041 | 0.69 | 0.984327 |
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Target: 5'- cGCAccUGAAGUCCGUGgUCGuUGAaCACg -3' miRNA: 3'- -UGU--ACUUCAGGCACaAGUuGCUgGUG- -5' |
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| 13432 | 3' | -49.1 | NC_003409.1 | + | 9867 | 0.7 | 0.9588 |
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Target: 5'- cCGUGAAGguggCCGUGaUCucguUGGCCACa -3' miRNA: 3'- uGUACUUCa---GGCACaAGuu--GCUGGUG- -5' |
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| 13432 | 3' | -49.1 | NC_003409.1 | + | 111086 | 0.75 | 0.808955 |
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Target: 5'- cGCGUGAucGGugccgagacgggcuUCCGUGggUCAAUGACCGCg -3' miRNA: 3'- -UGUACU--UC--------------AGGCACa-AGUUGCUGGUG- -5' |
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| 13432 | 3' | -49.1 | NC_003409.1 | + | 107864 | 1.11 | 0.008855 |
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Target: 5'- aACAUGAAGUCCGUGUUCAACGACCACg -3' miRNA: 3'- -UGUACUUCAGGCACAAGUUGCUGGUG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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