Results 1 - 5 of 5 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
13432 | 5' | -51.5 | NC_003409.1 | + | 106253 | 0.66 | 0.985627 |
Target: 5'- uUGCGAUUggGGAGGUgGuUGGAaUGUGg -3' miRNA: 3'- cACGCUAG--UCUCCAgC-GCCUaAUACa -5' |
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13432 | 5' | -51.5 | NC_003409.1 | + | 98729 | 0.67 | 0.971686 |
Target: 5'- -gGUGuUCAGGGGUUGgCGGAU-GUGUu -3' miRNA: 3'- caCGCuAGUCUCCAGC-GCCUAaUACA- -5' |
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13432 | 5' | -51.5 | NC_003409.1 | + | 17688 | 0.68 | 0.954176 |
Target: 5'- gGUaCGGUaaCAGAGGUCGCGGAa----- -3' miRNA: 3'- -CAcGCUA--GUCUCCAGCGCCUaauaca -5' |
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13432 | 5' | -51.5 | NC_003409.1 | + | 22139 | 0.7 | 0.893582 |
Target: 5'- cUGCGGUCAcGGGGgCGUGGAUUuuuUGg -3' miRNA: 3'- cACGCUAGU-CUCCaGCGCCUAAu--ACa -5' |
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13432 | 5' | -51.5 | NC_003409.1 | + | 107899 | 1.08 | 0.007556 |
Target: 5'- gGUGCGAUCAGAGGUCGCGGAUUAUGUg -3' miRNA: 3'- -CACGCUAGUCUCCAGCGCCUAAUACA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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