Results 21 - 23 of 23 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
13433 | 3' | -52.3 | NC_003409.1 | + | 52464 | 0.76 | 0.550496 |
Target: 5'- -uUUCCGCGUCgCGAGGAauggcgACAGGCACg -3' miRNA: 3'- gcAGGGUGCAG-GUUUCUa-----UGUCCGUG- -5' |
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13433 | 3' | -52.3 | NC_003409.1 | + | 99585 | 0.77 | 0.489789 |
Target: 5'- gGUCCCGCG-CCAAccaAUACAGGCAUu -3' miRNA: 3'- gCAGGGUGCaGGUUuc-UAUGUCCGUG- -5' |
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13433 | 3' | -52.3 | NC_003409.1 | + | 107958 | 1.1 | 0.005064 |
Target: 5'- aCGUCCCACGUCCAAAGAUACAGGCACc -3' miRNA: 3'- -GCAGGGUGCAGGUUUCUAUGUCCGUG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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