Results 1 - 9 of 9 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 13433 | 5' | -50.9 | NC_003409.1 | + | 60691 | 0.66 | 0.990483 |
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Target: 5'- ----aCCACcggaagcgacgcuGUCCGGACGgcuugaCGAUCGUCu -3' miRNA: 3'- acuaaGGUG-------------UAGGCCUGC------GUUAGCAG- -5' |
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| 13433 | 5' | -50.9 | NC_003409.1 | + | 134643 | 0.66 | 0.98615 |
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Target: 5'- uUGGUUaaUACAagCGGACGCAcgCGUUg -3' miRNA: 3'- -ACUAAg-GUGUagGCCUGCGUuaGCAG- -5' |
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| 13433 | 5' | -50.9 | NC_003409.1 | + | 78916 | 0.67 | 0.98022 |
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Target: 5'- aGAcccgUCCGuuUCCGcGAUGCAuUCGUCa -3' miRNA: 3'- aCUa---AGGUguAGGC-CUGCGUuAGCAG- -5' |
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| 13433 | 5' | -50.9 | NC_003409.1 | + | 68590 | 0.67 | 0.98022 |
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Target: 5'- ---gUCCACAgucucacgCCGGugGCGagucaaauaGUCGUUg -3' miRNA: 3'- acuaAGGUGUa-------GGCCugCGU---------UAGCAG- -5' |
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| 13433 | 5' | -50.9 | NC_003409.1 | + | 45495 | 0.68 | 0.963006 |
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Target: 5'- cUGAUcaugUCCACcgUCGGGCGUA--UGUCg -3' miRNA: 3'- -ACUA----AGGUGuaGGCCUGCGUuaGCAG- -5' |
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| 13433 | 5' | -50.9 | NC_003409.1 | + | 87375 | 0.71 | 0.881719 |
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Target: 5'- aUGGUUUCGCAUacCCGGGCGCGGgggCGc- -3' miRNA: 3'- -ACUAAGGUGUA--GGCCUGCGUUa--GCag -5' |
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| 13433 | 5' | -50.9 | NC_003409.1 | + | 104321 | 0.71 | 0.874282 |
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Target: 5'- ---gUCCAaagCCGGA-GCAAUCGUCg -3' miRNA: 3'- acuaAGGUguaGGCCUgCGUUAGCAG- -5' |
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| 13433 | 5' | -50.9 | NC_003409.1 | + | 45245 | 0.73 | 0.797627 |
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Target: 5'- gGGgcgCCACGUCCaGGACGUGucucUCGUCa -3' miRNA: 3'- aCUaa-GGUGUAGG-CCUGCGUu---AGCAG- -5' |
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| 13433 | 5' | -50.9 | NC_003409.1 | + | 107993 | 1.11 | 0.005932 |
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Target: 5'- cUGAUUCCACAUCCGGACGCAAUCGUCg -3' miRNA: 3'- -ACUAAGGUGUAGGCCUGCGUUAGCAG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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