Results 1 - 9 of 9 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 13434 | 5' | -52.3 | NC_003409.1 | + | 39162 | 0.66 | 0.972792 |
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Target: 5'- uGCAGGCCAUCg---CUcUCAUCGUAg -3' miRNA: 3'- uCGUCUGGUAGuucaGGuAGUGGCAU- -5' |
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| 13434 | 5' | -52.3 | NC_003409.1 | + | 94734 | 0.66 | 0.969829 |
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Target: 5'- cAGCAGGCCgAUCGAGgCCGUCgauaucagggugGCCa-- -3' miRNA: 3'- -UCGUCUGG-UAGUUCaGGUAG------------UGGcau -5' |
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| 13434 | 5' | -52.3 | NC_003409.1 | + | 51851 | 0.66 | 0.966641 |
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Target: 5'- cGUGGACCGUugCAGGUCC-UgACCGg- -3' miRNA: 3'- uCGUCUGGUA--GUUCAGGuAgUGGCau -5' |
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| 13434 | 5' | -52.3 | NC_003409.1 | + | 43342 | 0.66 | 0.962868 |
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Target: 5'- gGGCcucaaaaAGGCCGUCAAGUCUGaCAUgGUAu -3' miRNA: 3'- -UCG-------UCUGGUAGUUCAGGUaGUGgCAU- -5' |
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| 13434 | 5' | -52.3 | NC_003409.1 | + | 8255 | 0.67 | 0.95567 |
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Target: 5'- cAGUcGACCGUCAGGUCCu--GCCa-- -3' miRNA: 3'- -UCGuCUGGUAGUUCAGGuagUGGcau -5' |
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| 13434 | 5' | -52.3 | NC_003409.1 | + | 132545 | 0.67 | 0.951525 |
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Target: 5'- uGgAGGCCAUCAcuccGUCCAUgGCCa-- -3' miRNA: 3'- uCgUCUGGUAGUu---CAGGUAgUGGcau -5' |
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| 13434 | 5' | -52.3 | NC_003409.1 | + | 107448 | 0.67 | 0.951525 |
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Target: 5'- cAGCAGGCguUCAAGgauUUCAacuUCGCCGUc -3' miRNA: 3'- -UCGUCUGguAGUUC---AGGU---AGUGGCAu -5' |
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| 13434 | 5' | -52.3 | NC_003409.1 | + | 34741 | 0.68 | 0.932393 |
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Target: 5'- gAGCuGGACCGgcuuguUCAGGUCCAuacUCACgCGUc -3' miRNA: 3'- -UCG-UCUGGU------AGUUCAGGU---AGUG-GCAu -5' |
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| 13434 | 5' | -52.3 | NC_003409.1 | + | 108335 | 1.07 | 0.00649 |
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Target: 5'- gAGCAGACCAUCAAGUCCAUCACCGUAu -3' miRNA: 3'- -UCGUCUGGUAGUUCAGGUAGUGGCAU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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